- 學位論文
大鼠脊髓損傷之中樞神經再生之早期評估----以維他命B12作為急性受術之填充生長因子
Early evaluation of central nervous regeneration in rat spinal cord injury model with vitamin B12 as a growth factor during the surgery of acute stage
摘要
脊髓損傷造成的癱瘓對於患者是一輩子的折磨，至今尚未有一個標準的方法可以有效地治療。許多人嘗試注射各種藥物，且使用各種組織染色方式以觀察變化，企圖達到更好的進展，但恢復效果仍十分有限。由於注射維他命B12運用在周邊神經具有恢復神經生長的療效，因此本論文以誘發之電生理反應為依據，輔以組織染色切片，藉以觀察維他命B12對於脊髓損傷的治療作用。 為了達到此目的，我們設計於T8至T9之脊椎高度傷害大鼠單側背索（dorsal funiculus）做為形成脊髓損傷之模型，並設計了一簡單之雙通道微電極，以長期量測視丘內神經元之反應。這些實驗大鼠在存活一至三個月後犧牲，並取背索之電顯厚片在甲基藍染色後觀察脊髓背索面積和軸突數量的變化。 實驗結果顯示，在電生理訊號方面，有注射維他命B12之大鼠在傷害後第一週至第二週內有回升的現象。另外，在組織切片部分，長達三個月的時間下，沒有注射維他命B12之大鼠的背索面積，比起有注射維他命B12之大鼠還要少很多。從上述兩個面向的觀察中，得知維他命B12對於受傷之脊髓具有延緩退化甚至修復之高度可能性。
並列摘要
Paralysis caused by spinal cord injury (SCI) is a torture for patients in a life span. There is no way to cure paralysis nowaday. Many people managed to make a progress upon this through injecting various medicine. However, the recovery is still limited. Therefore, this study attempted to observe the effect of vitamin B12 on SCI rats by electrophysiological recordings, and subsidiarily supported by histological examinations. For chronic recordings, 2-channel micro-wire electrode was embedded to the scalp of the rat to record the evoked neuronal responses reaction of neurons in thalamus. Then, one side of the dorsal column of the rat spinal cord between the T8 and T9 segment was lesioned to induce the SCI model. The results showed that the signals of rats injected with vitamin B12 bounced back from week 1 to week 2 after SCI. Morphologically, after three months the areas of dorsal funiculus of non-treated rats were much less than those of rats which were treated with B12. Based on the preliminary results, vitamin B12 might have the high possibility of degeneration retarding or even recovery to SCI.