表面聲波式微液珠控制系統之研製及其於核酸增幅反應之應用

Development of a Surface Acoustic Wave Based Micro-Droplet Control System and its Application of Nucleic Acid Amplification

何亞倫(Ya-Lun Ho)

指導教授 ： 吳政忠

國立臺灣大學/工學院/應用力學研究所/碩士(2010年)

https://doi.org/10.6342/NTU.2010.01446

摘要

本論文利用表面聲波（surface acoustic wave）元件所設計之自動化微液珠操縱平台，結合電阻式微加熱器及溫度感測器，研製出可應用於核酸增幅反應（amplification of nucleic acid）之微液珠控制系統。
微液珠操縱平台主要是藉由斜交指叉電極（SFIT）所激發之表面聲波通過液態與固態之界面時，轉換成之滲漏表面波（leaky Rayleigh Wave）與縱波，並產生聲射流效應（acoustic streaming）以驅動微液珠。同時，斜交指叉電極激發寬頻表面聲波之頻率響應，亦可進行微液珠的位置偵測。利用比例積分控制器（PI controller）結合驅動與偵測之斜交指叉電極，可達到微液珠自動控制的目的。此外，為降低驅動微液珠所需之能量，並克服進行生醫反應時所使用之礦物油不易在微液珠操縱平台上驅動的缺點，本論文利用全氟矽烷（perfluoroalkylsilane）及四乙氧基矽烷（tetraethoxysilane）來製備疏水薄膜進行表面改質，其結果可有效提昇礦物油在微液珠致動平台表面之接觸角，並使微液珠易於驅動。此疏水薄膜除了可讓微液珠更易於驅動，亦具有液體不易殘留之特性，使進行核酸增幅反應之微液珠控制系統達到重複使用的性能。
利用此具有自動化位置控制及溫度控制功能之微液珠控制系統，已可成功進行核酸增幅反應，且有效降低生醫檢體之使用量及成本，同時增進反應之效率。此微液珠控制系統適合應用於發展各類型生醫檢測之實驗室晶片。

關鍵字

微液珠 ； 核酸增幅反應 ； 斜交指叉電極 ； 表面聲波 ； 比例積分控制器

並列摘要

In this thesis, an automatic micro-droplet control system applied to amplification of nucleic acid is accomplished by the combination of a surface acoustic wave (SAW) device, micro-heaters, micro-sensors of temperature, and a PI controller.
The SAW device constituted of slanted finger interdigital transducers (SFITs) is utilized to actuate the micro-droplet by the acoustic streaming and to detect the micro-droplet by the frequency responses of the SAWs. With the development of PI controller, the micro-droplet can be manipulated automatically. In order to reduce the driving power and manipulate the mineral oil which is necessary for the reaction of the nucleic acid amplification, a perfluoroalkylsilane (PFAS) and tetraethoxysilane (TEOS) hydrophobic film is utilized for surface modification. Furthermore, with the PFAS/TEOS hydrophobic film, the cross-contamination can be prevented, and the micro-droplet control system is reusable for different DNAs and reagents.
Utilizing the developed micro-droplet control system, the amplification of nucleic acid can be successfully achieved. The consumption of the biomedical reagent and the production cost of devices can be reduced, and the efficiency of reaction can be improved. The developed micro-droplet control system is suitable for various types of lab-on-a-chip system in biomedical fields.

並列關鍵字

Micro-droplet ； Nucleic acid amplification ； SFIT ； SAW ； PI controller

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