比較橈足類與豐年蝦餵食點帶石斑魚苗之發生與基因表現

Comparison of gene expression profile and development of red spotted grouper (Epinephelus coioides) larvae fed with copepod and Artemia

高瑞國(Jui-Kuo Kao)

指導教授 ： 陳弘成

共同指導教授 ： 張繼堯(Chi-Yao Chang)

國立臺灣大學/生命科學院/漁業科學研究所/碩士(2009年)

https://doi.org/10.6342/NTU.2009.10305

摘要

石斑魚是台灣重要的高經濟養殖魚種，其魚苗培育中期的餌料生物多以豐年蝦與橈足類為主，為了解不同餌料生物對魚苗發育之影響，我們首先以離水測試餵食不同餌料生物之石斑魚苗其抗緊迫之存活率，結果得豐年蝦餵食組8.13%、超級熊克滋養豐年蝦餵食組72.28%、橈足類餵食組98.21%。進一步探討這些餵料生物與所餵食石斑魚魚的脂肪酸組成。在餌料生物方面，豐年蝦含，DHA 0.22%，EPA 1.80%，ARA 0.48%，超級熊克滋養豐年蝦含DHA 4.53%，EPA 5.12%，ARA 0.66%，橈足類含DHA 6.30%，EPA 8.48%，ARA 1.67%；在魚苗方面，餵食豐年蝦組DHA 1.66%，EPA5.24%，ARA 3.28%，餵食超級熊克滋養豐年蝦組DHA 7.14%，EPA 6.44%，ARA 3.81%，餵食橈足類組DHA 15.47%，EPA 7.13%，ARA 1.57%。其中只有餵食橈足類組在14天餵食期結束後完成魚苗變態。此結果顯示，食物鏈中的高度不飽和脂肪酸含量對於石斑魚苗的發育、變態、抗緊迫存活率有很高的影響。以微陣列分析餵食橈足類與豐年蝦之基因表現差異，發現2.5倍以上促進表現的基因有253 個，抑制表現的基因有123 個，這些差異性表現基因包括醣解作用相關的基因如：muscle-beta-nolase 3、pyruvate kinase、muscle-phosphoglycerate mutase 2-1、glyceraldehyde-3-phosphate dehydrogenase及Fructose-bisphosphate aldolase A等；與核酸合成，細胞調控相關的基因如：Nucleoside diphosphate kinase B、 nucleoside diphosphate kinase-Z1；與結構相關的基因如：lily-type 、lectinmuscle-specific beta 1 integrin binding protein 2及similar to Histone H1.5。

關鍵字

石斑魚, 高度不飽和脂肪酸

並列摘要

Grouper is important culture fish with high economic value in Taiwan, the major living feed during the middle stage of grouper fry culture are artemia and Copepods. To understand the effect of different living feed on fish fry development, we first test the anti-stress survival rate that remove from water of grouper fry fed with different living feed, the survival rate of the group fed with artemia was 8.13%, the group fed with artemia enriched with super SELCO was 72.28%, and the group fed with Copepods was 98.21%. Then we further investigated the fatty acid composition of living feed and grouper. In the living feed, artemia contains DHA 0.22%, EPA 1.80%, ARA 0.48%; artemia enriched with super SELCO contains DHA 4.53%, EPA 5.12%, ARA 0.66%; Copepods contain DHA 6.30%, EPA 8.48%, ARA 1.67%. And in fish fry, the group fed with artemia contains DHA 1.66%, EPA 5.24%, ARA 3.28%, fed with artemia enriched with super SELCO contains DHA 7.14%, EPA 6.44%, ARA 3.81%, and the group fed with Copepods contains DHA 15.47%, EPA 7.13%, ARA 1.57%. In which, only the group fed with Copepods completes fry metamorphosis after 14 days of feeding period. The results showed that the content of highly unsaturated fatty acid in food chain has significant influence on the development, metamorphosis and anti-stress survival rate of grouper fry. We also used microarray to analyze the differential gene expression of fry fed with artemia and copepods, and found that there were 253 genes with up-regulated and 123 genes with down-regulated. These differentially expressed genes include glycosylation related genes: muscle-beta-nolase 3, pyruvate kinase, muscle-phosphoglycerate mutase 2-1, glyceraldehyde-3-phosphate dehydrogenase and fructose-bisphosphate aldolase A; nucleic acid synthesis and cellular regulatory related genes: nucleoside diphosphate kinase B and nucleoside diphosphate kinase-Z1; structural gene：lily-type, lectinmuscle-specific beta 1 integrin binding protein 2 and similar to Histone H1.5.

並列關鍵字

grouper, DHA, EPA

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