製備MCP-1功能化之含奈米金棒有機金屬框架(MCP-1-GNR@MIL-100)利用免疫細胞傳遞並標靶腫瘤進行光熱治療

MCP-1 Functionalized, Gold Nanorods@Metal-Organic Frameworks (MIL-100(Fe)) Core-Shell Nanoparticles for Immune Cell Drug Delivery and Photothermal Therapy

簡瑋呈(Wei-Cheng Chien)

指導教授 ： 吳嘉文

國立臺灣大學/工學院/化學工程學研究所/碩士(2020年)

https://doi.org/10.6342/NTU202001571

摘要

腫瘤內的缺氧區因為血管缺乏與氧氣含量低導致癌症治療的功效不足進是造成癌症容易復發的原因之一。巨噬細胞具有突破血管屏障抵達缺氧區的能力，藉由巨噬細胞傳遞藥物以標靶腫瘤是一相當具有潛力的傳遞方式
我成功將單核球趨化蛋白-1(MCP-1)與有機金屬框架(MIL-100)修飾於光熱材料奈米金棒(GNR)上，以吸引巨噬細胞的吞噬與材料的生物相容性同時具有標靶腫瘤的能力。從穿透式電子顯微鏡(TEM)、紫外光可見光光譜儀(UV-Vis)與傅立葉轉換紅外光(FTIR)等結果證實我成功製備出MCP-1/GNR@MIL-100，從生物毒性結果看出經由MIL-100修飾後的生物毒性大幅改善。藉由細胞遷移實驗與光學顯微鏡的觀察可以看出經由MCP-1的修飾可以提高巨噬細胞的吸引與吞噬，同時巨噬細胞在攜帶材料後也具有標靶腫瘤的能力。在經過光熱治療後癌細胞的存活率僅剩20%，這些都證實此材料具有很好的潛力應用在生物體內進行癌症治療。

關鍵字

奈米金棒 ； 有機金屬框架 ； 光熱治療 ； 單核球趨化蛋白-1 (MCP-1)

並列摘要

The insufficient blood vessel and oxygen concentration in hypoxia induce the low efficiency of anti-cancer therapeutics and easy relapse. Macrophage has the ability to cross the blood-vessel barriers to reach the hypoxic region of tumors, so target the tumor by macrophage delivery has been a good potential in tumor targeting.
In this work, I successfully modify monocyte chemoattractant protein-1 (MCP-1) and iron-based metal-organic framework (MIL-100) on phototheraml agent, gold nanorods (GNR) (i.e. MCP-1/GNR@MIL-100), in order to increase cellular uptake and biocompatibility. TEM, UV-Vis, and FTIR show successfully synthesis of MCP-1/GNR@MIL-100, and the biocompatibility was improved after MIL-100 modification. Transwell assay show macrophage attractant and material uptake show 1.5 time increase after MCP-1 functionalization and also indicate the macrophage have tumor targeting ability. The in vitro photothermal therapy result show the cell viability decreased to 20%, it all show the materials have good potential in cancer therapy in in vivo experiment.

並列關鍵字

Gold nanorods ； MIL-100 ； photothermal therapy ； monocyte chemoattractant protein-1 (MCP-1)

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