The spray nozzle with different frame holders and fuels are investigated. The experimental are mainly compose of Diesel and Bio-Diesel and the frame status such as color and length are fully studied. The results show that even in the same experimental environment, the distinct emulsion such as Diesel, Bio-diesel, water, and ethanol would lead to various spray combustion phenomena. These findings also reveal that the high frame holder will promote combustion efficiency with the fuels which tend to evaporate and burn. Otherwise, the low high frame holder will increase the combustion ability with the fuels which are hard to vaporized and ignited.