被動閥門在微流道系統中針對於LAMP的應用

Using Passive Flow Valves in a Micro-Fluidic System for LAMP Application

張宏誠(Hung-Cheng Chang)

指導教授 ： 顏家鈺

國立臺灣大學/工學院/機械工程學研究所/博士(2014年)

https://doi.org/10.6342/NTU.2014.01649

摘要

無資料

關鍵字

科氏力 ； 離心力 ； 毛細力 ； 環形核酸增幅法 ； 高分子發光二極體

並列摘要

This research presents the design of the passive flow valves in a microfluidic loop-mediated isothermal amplification (LAMP) system. It is possible to manipulate the microfluidic flow by carefully changing the balance among various participating forces including the Coriolis force, the centrifugal force and the capillary force. The Coriolis force is perpendicular to the tangential velocity, thus one can use slanted channels to change the flow direction. Changing disk spinning direction and velocity can change the balance between the capillary force and the centrifugal force. A multi-stage channel design was then implemented for a lab-on-a-disk biomedical diagnostic LAMP system. The lab-on-a-disk system consists of a servo system for centrifugal pumping and a heater for temperature control. To minimize the evaporative loss from the LAMP process, the system integrates the multistep reactor with heating capability to allow real-time LAMP execution. A polymer light emitting diode (PLED) section deposited on the disk then enables the turbidity test readout.

並列關鍵字

Coriolis force ； Centrifugal force ； LAMP ； Capillary force ； PLED

參考文獻

[1] Madou, M., Kellogg, G. “The LabCD, A centrifuge-based microfluidic platform for Diagnostics”, SPIE Proceedings, 1998.

[14] Schembri, C. T., Burg T. L., Kopfstill A.R., Shea L.R., Brayin, B., “Centrifugation and Capillarity Integrated into A Multiple Analyte Whole-Blood-Analyzer,” Journal of Automatic Chemistry, vol. 17, no. 3, pp. 99-104, 1995.

[16] Lee, L. J., Madou, M. J., Koelling, K. W., Daunert, S., Lai, S., Koh, C. G., Juang, Y., Lu Y.; Yi L., “Design and Fabrication of CD-Like Microfluidic Platforms for Diagnostics: Polymer-Based Microfabrication,” Biomedical Microdevices, vol. 3, no. 4, pp.339-351, 2001.

[18] Lai, S., Wang, S., Luo, J., Lee, L. M., Yang, S. T., Madou, M. J., “Design of a Compact Disk-like Microfluidic Platform for Enymelinked Immunosorbent Assay,”, vol. 76, no. 7, pp. 1832-1837, 2004.

[19] Puckett, L. G., Dikici, E., Lai S., Madou, M., Bachas, L. G., Daunert, S., “Investigation into the Applicability of the Centrifugal Microfluidics Platform for the Development of Protein-Ligand Binding Assays Incorporating Enhanced Green Fluorescent Protein as a Fluorescent Reporter,” Analytical Chemistry, vol. 76, no. 24, pp. 7263-7268, 2004.