自動感測實驗鼠隻豐富系統相關活體識別數據與實驗分析應用

Autosensing Lab-Mice Enrichment System with Vivimetric Data and Trial Analyses

陳重瑞(Tsung-Jui Chen)

指導教授 ： 高成炎

共同指導教授 ： 張春梵(Chun-Fan Chang)

國立臺灣大學/電機資訊學院/資訊工程學研究所/碩士(2010年)

https://doi.org/10.6342/NTU.2010.01987

摘要

建置實驗動物福利人道管理系統，可以避免人工作業量測動物生理參數造成的緊迫，有效取得近無緊迫型式的量測動物活體數據，提升實驗數據的可靠性與工作人員的安全性。本項論文建置實驗小鼠的自動監控感測豐富環境硬體系統，對應建置圖型使用介面的即時控管與統計探採軟體系統。原型化的整合硬體軟體豐富環境系統每日感測超過千筆量測數據包括活體識別、生物參數、行為事件、與環境參數，實作高效過濾演算獲得實驗小鼠的合理有效數值進行後續相關數值統計與資料探採。個別活體實驗小鼠藉由皮下植入無線射頻識別標籤歸屬原型系統蒐集相關籠組位置系統模組的體溫、體重、與跑速等生物參數，追蹤呈現個別小鼠的整體系統活動路徑以及成對小鼠的個別位置佔用更替繼而延伸演算整群小鼠的社群層級壓力排名。本項論文設計前導測試初步評估此項原型化的整合硬體軟體實驗小鼠豐富環境系統，全程31天實驗期間放置實驗小鼠10隻，在於系統既有內部緊迫的社群層級壓力，施加外部緊迫的藥物攝取型咖啡飲用與噪音干擾型廣播放送，實施方式分別包括夜間日間、系列稀釋、與系列音量。實驗結果顯示豐富環境系統實驗小鼠體重無法展現強勢弱勢相關差異，或因豐富環境減低資源競爭的生存壓力；實驗結果顯示內部緊迫的整群實驗小鼠的社群層級壓力排名，以及施加外部緊迫的社群層級數據融合變異，期望藉由心律變異驗證社群層級的融合變異以及校正實驗數據的系統偏差。

關鍵字

實驗小鼠 ； 射頻識別 ； 自動監測 ； 社群層級 ； 心律變異

並列摘要

The deployment with humane management system of lab mice welfare may overcome manipulation stress by lab staff onto measuring animal physiological index in order to obtain effective data of live subject measurements at nearly nonstressful status which in turn improves the reliability of experimental data and the safety of staff. With realized lab mice autosensing enrichment system in this thesis, compatible software system implements graphic user interfaces of online control panel and data mining. The prototype lab mice enrichment system of integrated hardware and software retrieve more than 1,000 data entries daily with vivimetric identification, biological parameters, behavioral events, and environmental parameters. The in-house algorithm of efficient filter effectively fetches adequate value for subsequent data statistics and mining. The vivimetric identifications based on implanted subcutaneous radio frequency identification (RFID) tags attributes lab mice biological parameters including body temperature, body weight, and running wheel speed. The tracking on activity paths of individual lab mouse and occupancy alternation of overall mice pairs extends computations to social rankings among lab mice as for social interaction stress. The designed pilot trial for 31-day duration preliminarily assesses on 10 lab mice within the prototype lab mice enrichment system of integrated hardware and software upon external stress introductions. In addition to internal stress of social ranking stress, the external stress introduction upon coffee drinking and noise radio broadcasting may be applied in formats including daytime/nighttime, serial coffee dilution, and serial sound volume. The results showed that body weight of mice in the enrichment system fails to show the relationship among dominant and submissive subjects which may be due to that the enrichment system reduces the survival pressure upon resources competition. The data mining results of pilot trial reveals the internal stress with social stress ranking among overall lab mice and as well the external stress with ranking data fusion on social rankings variations. With subsequent analysis on lab mice heart rate variability (HRV) with empirical mode decomposition (EMD) to more accurate baseline for trial data interpretations, the data fusion variations of social rankings may be verified and the systemic deviation of experimental data may be calibrated upon stress introduction.

並列關鍵字

Lab Mice ； Radio Frequency Identification ； Automated Surveillance ； Social Ranking ； Heart Rate Variability

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