複合式微結構平台引導細胞遷移

Composite Microstructural Substrate Induces Cell Migration

張維仁(Wei-Jen Chang)

指導教授 ： 趙本秀

國立臺灣大學/工學院/醫學工程學研究所/碩士(2011年)

https://doi.org/10.6342/NTU.2011.01956

摘要

本論文的目的為利用複合式平台來研究細胞型態以及行為。複合結構式平台是由兩層不同楊式模數的材料所組成，使用材料包含膠原蛋白以及PDMS，上層為硬度較小的膠原蛋白，下層為硬度較大且有圓柱陣列結構的PDMS， 藉由下層圓柱狀結構影響上部較軟層膠原蛋白的機械性質，期使培養在膠原蛋白上方之細胞感受到下方基質幾何的不同而有所改變。首先，使用有限元素法分析去模擬可能改變的機械性質以及使用原子力顯微鏡去測量真實的有效軟硬度模數。利用此系統，本論文研究內容包括：複合結構平台上層的膠原蛋白厚度及圓柱間距離對間葉幹細胞遷移及型態的影響、間葉幹細胞在複合結構平台之三維環境中之反應、以及比較分化及未分化細胞（前十字韌帶纖維母細胞及間葉幹細胞）在複合結構平台上的遷移及型態。本研究發現細胞會往圓柱上方較薄層的膠原蛋白表面移動，這說明了細胞可感受到複合式平台中的內部結構變化，並且不同種類的細胞對於感受複合式平台中的內部結構的程度有所不同。

關鍵字

細胞爬行 ； 軟硬度

並列摘要

The thesis used the laboratory-designed composite substrate microstructure to study the cell morphology and behavior.
This composite substrate device offers a homogeneous chemical property of 2D and 3D environment, and the inner geometric and microstructural design can be easy and precious to manipulate.
First, we used the finite element method to simulate the mechanical property of composite substrate and atomic force microscopy(AFM) to measure the effective modulus of composite substrate.
There are four research topics in the thesis, including effect of collagen thickness and spacing distance between micropillars on human mesenchymal stem cells (hMSCs) migration and morphology, and effect of three-dimension environment on hMSCs migration and morphology, and effect of composite substrate microstructure on anterior cruciate ligament cells (ACL) migration and morphology.
In our study, most cells migrated toward the composite thin gel on the micropillars, and this implies cells seem like to have potential to sense the underlying structure of our composite substrate. In addition, different cell types have different sensitivity for the underlying structure of our composite substrate.

並列關鍵字

Cell migration ； Stiffness

參考文獻

Chapter 5 References

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