- 學位論文
神經訊號即時量測 -平面多通道電極陣列與訊號擷取系統之建構
Real-time measurement of neuron signal -the development of two dimensional multi-electrode array chip and signal measuring system
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摘要
本研究使用半導體技術建立一神經細胞群體訊號量測之平台,從平面多通道電極晶片之設計製作,到濾波放大電路及訊號擷取與記錄系統之研製,最後並對系統進行測試與驗證 本研究之二微晶片於無塵室內使用光顯影術,薄膜沉積製作.共有使用透明導電材料-銦錫氧化物(Indium tin oxide)電極晶片與金薄膜電極晶片兩種.晶片包含玻璃基底,ITO電極與金電極以及二氧化矽絕緣層.ITO電極晶片共有10通道數,每個通道長為500μm寬為200nm厚度為150nm彼此間距為200nm.金電極晶片共有18個電極通道數以9*2矩陣方式排列,長為200μm寬為40μm厚度為100nm彼此間距為200μm.之後經由探針將訊號接取到訊號擷取系統. 訊號擷取模組之設計包含儀表放大器,二階fliege notch filter,四階MFB高低通濾波器,後端放大器,供電電池.模組設計之放大頻率為一千倍,此模組可以有效降低雜訊影響,搭配市售之類比數位轉換介面與LabVIEW程式語言所開發之人機介面以完成整個細胞訊號觀測平台.最後以老鼠胚胎之神經細胞對整個系統進行生理電氣訊號偵測之驗證.
並列摘要
In this research, we set up a measurement platform for the recording of neuron ensemble by using semi-conductor technology. A 2D multiple electrode array chip , interface circuits, and signal recording system were designed and implemented. The MEA chips are fabricated in clean room using photolithography and thin film deposition. We fabricate two kinds of MEA chips. One is ITO electrode array chip, the other is gold electrode array chip. These chips are composed of a glass substrate, ITO or gold thin film electrode and silicon dioxide insulation layer. ITO chips are composed of ten electrodes .The length of ITO electrode is 500μm, the width is 200nm and the thickness is 150nm. Gold chips are composed of eighteen electrodes arranged in a 9*2 matrix. The length of gold electrode is 200μm ,the width is 40μm and the thickness is 100nm.Connecting these chip to probe enabling the transmission of the detected neuronal signal to signal processing system. This signal processing system includes an interface circuit which is composed an instrument amplifier, a fliege notch filter, a MFB high pass filter ,a MFB low pass filter and an analog to digital converter card with LabVIEW interface. Bio-electrical signals of rat neuron from embryo can be successfully detected from this system.
參考文獻
[18] 陳建銘 “平面式多通道微電極陣列晶片系統之研發”國立台灣大學碩士論文 2004
[19] 盧妙月 “64通道微電極量測系統之研製” 國立台灣大學碩士論文 2005
[1] 曾忠玲,郭東泰,駱榮欽 “研製一套改進之高SNR侵入式多層腦部生醫訊號擷取、紀錄與分析系統” 國立台北科技大學電腦與通訊研究所
[3] Fu-Zen Shaw , Chen-Tung Yen , Ruei-Feng Chen “A simple and effective process for noise reduction of multichannel cortical field potential recordings in freely moving rats” Journal of Neuroscience Methods 124 (2003), PP 167-174
[4] Jonathan Ericksona, Angela Tookerb, Y.-C. Tai b, Jerome Pinec “Caged neuron MEA: A system for long-term investigation of cultured neural network connectivity”