具離子電泳功能並可長期植入之神經探針

在現代的電生理的研究中，常使用神經探針以記錄腦神經電位。本研究使用微機電系統技術，設計與製造新型神經探針，依據其功能的不同，本研究的神經探針可分為兩種：完全以聚-對二甲苯包覆之神經探針與具有微流道之神經探針。對長期植入生物體內的微元件而言，生物相容性的考量是不可或缺的一項指標，為了讓以矽為基材的神經探針更適合進行長期植入的實驗，本研究成功以具有高度生物相容性的高分子材料：「聚-對二甲苯」將探針完全包覆，本研究發展創新的微元件全包覆技術，將微元件整體包覆聚-對二甲苯並且可允許特定位置開洞，突破微機電製程技術以往僅能進行微元件單面的包覆處理。將神經探針整合微流道，使得探針不但可以傳輸微流體以刺激生物組織，還可以觀察組織受刺激前後的神經電位變化。本研究整合二條微流道於一神經探針上，相對於之前文獻上的相關的研究，本研究省去利用外接的注射幫浦驅動微流體，而改以在微流道前後端佈置電極，提供電壓下，可在微流道中產生離子電泳以驅動微流道內帶電之試劑，。本研究成功量測兩種神經探針的電氣規格。並以完全聚-對二甲苯包覆之神經探針植入大鼠的大腦皮質中進行動物實驗，可記錄到大鼠後腳受機械刺激所誘發之大腦神經訊號。具有微流道之神經探針上經酒精與純水測試後，確定其微流道(長7釐米、寬60微米)中無任何阻塞。

關鍵字

微機電；神經探針；微流道；生物相容性；聚-對二甲苯

並列摘要

Nowadays, neuroprobes are commonly used in electrophysiological studies. Novel neuroprobes are designed and implemented with Micro-Electro-Mechanical-System technology in this thesis. There are two kinds of neuroprobes designed: one is the completely parylene-coated and the other is that with micro-fluidic channels neuroprobes. Biocompatibility is essential for a device to be implanted in a living organism chronically. In order to make the silicon-based probes serve this purpose, the designed neuroprobes were completely coated with parylene a high polymer biocompatible material. The neuroprobe integrated with micro-fluidic channel can not only deliver agents to stimulate the tissues but also record the evoked neural potentials. For each neuroprobe two micro-fluidic channels were integrated. Electrodes placed at the front and the end of micro-fluidic channel were applied with a voltage that can cause iontophoresis to drive the agents. We have measured the electrical specifications of two neuroprobes. Completely parylene-coated neuroprobe was implanted in the primary sensory cortex of a living rat and mechanical stimuli were applied on the contralateral hind paw. The evoked potentials was recorded successfully. We tested micro-fluidic channel neuroprobe both by alcohol and by water, and demonstrated that the micro-fluidic channels (7mm-long and 60µm-wide) could function correctly.

並列關鍵字

MEMS ； neuroprobe ； microfluidic channel ； biocompatibility ； parylene

參考文獻

[1] Jack W.Judy, "Microelectrode Technologies for Neuroengineered Systems," Proceedings of the 25th Annual lntemational Conference of the IEEE EMBS 2003, pp. 17-21.

[2] P. Norlin, M. Kindlundh, A. Mouroux, K. Yoshida, and U. G. Hofmann, "A 32-site neural recording probe fabricated by DRIE of SOI substrates," Journal of Micromechanics and Microengineering, vol. 12, no. 4, pp. 414-419, July 2002.

[3] C. Y. Xu, W. Lemon, and C. Liu, "Design and fabrication of a high-density metal microelectrode array for neural recording," Sensors and Actuators A-Physical, vol. 96, no. 1, pp. 78-85, Jan. 2002.

[4] G. Buzsaki, "Large-scale recording of neuronal ensembles," Nat. Neurosci., vol. 7, no. 5, pp. 446-451, May 2004.

[5] T. M. Pearce, J. J. Williams, S. P. Kruzel, M. J. Gidden, and J. C. Williams,"Dynamic control of extracellular environment in In Vitro Neural Recording Systems," IEEE Trans. on neural system and rehabilitation Eng, vol. 13, no. 2, pp. 207-211, June 1992.

國際替代計量

具離子電泳功能並可長期植入之神經探針

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