Bifidobacterium longum 為主要成份優酪乳對於大鼠腸道菌相之改善效果

The improvement effect of yogurt containing mainly Bifidobacterium longum on gut microbiota in rats

黃聖方(Sheng-Fang Huang)

指導教授 ： 潘子明

國立臺灣大學/生命科學院/生化科技學系/碩士(2017年)

https://doi.org/10.6342/NTU201701001

摘要

腸道菌相在體內扮演了許多角色，具有調節免疫、血脂、血糖等功能，也可以提供腸道細胞所需營養及能量，並可以協助宿主抵抗病原菌入侵。一旦菌相發生紊亂可能會導致疾病發生，而益生菌及發酵乳先前已有許多文獻證實其調節腸道菌相之功效。本研究使用含 Bifidobacterium longum 之發酵優酪乳作為樣品，每日管餵 SD 大鼠低、中、高三個劑量之 NY 優酪乳凍乾粉末，各組劑量分別為 2.2 g/kg BW、4.4 g/kg BW 及 8.8 g/kg BW (相當於含優酪乳 9.3 g/kg BW、18.6 g/kg BW 及 37.2 g/kg BW; 含 B. longum 則各為 3.40 × 104 cfu/kg BW、6.80 × 104 cfu/kg BW 及 1.36 × 105 cfu/kg BW)，探討不同劑量對於腸道菌相及糞便情況之改善效果。研究結果顯示管餵低、中、高劑量之優酪乳隨著餵食時間增加，皆可以提升糞便 Bifidobacterium spp. 與 Lactobacillus spp. 之菌數及降低盲腸及糞便內 Enterobacteriaceae 及 Clostridium perfringens 之菌數，顯示其具有改善腸道菌相之功能。此外餵食 NY 優酪乳亦可以提升腸道轉運速率、降低糞便 pH 值、增加糞便水分含量，並可以提高糞便及盲腸內容物之短鏈脂肪酸含量，藉此增進腸道健康。綜合以上結果，含 B. longum 之發酵優酪乳可以透過調節腸道菌相、改善糞便情況及腸道蠕動速率，以及增加短鏈脂肪酸含量來達到促進腸道健康之功效。

關鍵字

Bifidobacterium longum ； 腸道菌相 ； 腸道蠕動 ； 短鏈脂肪酸

並列摘要

Gut microbiota plays an important role in our body, it have several functions, such as immune regulation, blood lipid and blood glucose regulation, nutrients production and energy required for intestinal cells. And it provides a barrier to against pathogen invasion. Gut microbiota dysbiosis may lead diseases, as probiotics and fermented milk could rebalance gut microbiota in previous studies. In this study, using fermented yogurt containing Bifidobacterium longum as sample (NY yogurt) to understand the intestinal beneficial effect of different doses. Sprague-Dawley (SD) rats were orally administrated with 2.2 g/kg BW, 4.4 g/kg BW and 8.8 g/kg BW yogurt powder per day (i.e. 9.3 g/kg BW, 18.6 g/kg BW and 37.2 g/kg BW yogurt; 3.40 × 104 cfu/kg BW, 6.80 × 104 cfu/kg BW and 1.36 × 105 cfu/kg BW B. longum), respectively. This result shows that feeding B. longum contenting yogurt can increase fecal Bifidobacterium spp. and Lactobacillus spp. contents, and decrease the numbers of Enterobacteriaceae and Clostridium perfringens in cecum and feces. It displays that this yogurt has improvement effect on gut microbiota. In addition, feeding NY yogurt can increase intestinal transit rate, reduce fecal pH value, increase fecal moisture, and increase caecum and fecal short-chain fatty acid contents. Thereby, it shows the enhancing effects of intestinal health. Based on the above results, the fermented yogurt containing B. longum can improve fecal condition and intestinal peristalsis by adjusting gut microbiota, and enhancing the short-chain fatty acids to promote intestinal health.

並列關鍵字

Bifidobacterium longum ； gut microbiota ； intestinal peristalsis ； short chain fatty acid

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