The suspended particulate is one of the main indoor air pollutants. Generally, the removal methods of the suspended particulate can be classified to filtration and electrostatic collection technologies. The negative air ion (NAI) is a kind of low pollution material with electric charge. The purpose of this work is evaluating the NAI for removal of indoor suspended particulate. In this work, we used corona discharge to produce NAI (3000-5000 ions/cm3). Two sizes (0.3 and 0.03 μm) of NaCl were selected as the test aerosol to inject into the chamber which was manufactured form stainless steel to imitate indoor environment. We select four kinds of common indoor building materials (timber, wallpaper, of plastics, cement paint) to adhere to the surface of the wall inside the chamber. Four index was used to illustrate the characteristics of the particle removed by NAI, including removal efficiency, T50 (the half-life of particle concentration), Qi (the net efficiency of negative air ion) and reentrainment. The results showed that when the NAI was turned off, the 0.3 μm-particle removal efficiency of the plastics (26%) is highest and that of the stainless steel (10.2%) is lowest. For 0.03 μm-particle, the removal efficiency of cement paint (46.2%) and plastics (40.3%) are better than that of others, as well as that of the stainless steel (25.6%) is the lowest. While the NAI was turned on, the 0.3μm-particle removal efficiency of the plastics (68.2%) is largest, and that of the stainless steel (44.4%) is smallest. With 0.03μm particle, the removal efficiency of the timber(82.5%) is highest and that of the stainless steel (59.6%) is lowest. The results showed that when the NAI was turned off, the 0.3μm-particle T50 of the wallpaper(70.7 mins) is shortest, and that of the stainless steel(192.5 mins) is longest. For 0.03μm-particle, the T50 of the cement paint(33.6 mins) is shortest, and that of the stainless steel(67.3 mins) is longest. While the NAI was turned on, the 0.3μm-particle T50 of the timber(16.5 mins) is shortest, as well as that of the stainless steel (34 mins) is longest. For 0.03μm particle, the T50 of the timber(12 mins) is smallest and that of the stainless steel (22.4 mins) is longest. As regard the Qi of 0.3μm particle, the Qi of the timber(34.7 Lpm) is largest and that of the cement paint(14 Lpm) is smallest. For 0.03μm particle, the Qi of the timber(41.9 Lpm) is highest, as well as that of the stainless steel(20.5 Lpm) is lowest. Regard to reentrainment, the reentrainment of 0.3μm-particle was only observed of the wallpaper, and the level of the reentrainment is about 2.6%. The reentrainment of 0.03μm-particle was observed of the stainless steel, cement paint and wallpaper and the level of the reentrainment of them are 6.6%, 4.6% and 3.6%. The result of this study shows that the material of timber has the best removal efficiency while NAI was turned on, and that of the stainless steel has the lowest removal efficiency. As regard the size of particles, the removal efficiency of 0.03μm- particle is better than that of 0.3μm-particle while we turn on the NAI. Regard to T50, the T50 of 0.03 μm-particle is shortest than that of 0.3μm-particle and that of the timber is shortest, as well as that of the stainless steel is longest. As regard the reentrainment, the level of the reentrainment of 0.03μm particle is more obvious than 0.3μm.