非線性光學顯微技術於肺癌診斷分析及活體研究平台之應用

肺癌，國民十大死因之首，長久以來一直侵襲著世人們，為了將肺癌自十大死因中除名，當務之急就是能發展一套快速及有效的診斷系統，進而達到早期發現，有效治療的功效，於是乎運用非線性光學的特性，在肺癌診斷上開發出除了定性之外還有定量分析能力的系統，是本篇論文的主軸之一。在活體觀察平台作用之下配合我們所發展的癌症診斷系統，為奇妙生物體提供有別以往更接近細胞實際生長情境的全新體驗。雙光子顯微技術，一種全新的而有別於傳統顯微技術的發展，強化了各種應用的層面，由於雙光子顯微技術不需對組織檢體做切片，即能因其光學特性而達到如傳統切片般的效果，也因為如此，大大的縮短了檢體製造上所耗損的時間，及降低製作檢體時所造成的破壞。且傳統診斷的方式，其所著重者為形態上的差異及觀察，是屬定性上的分析，缺乏定量上的分析；而雙光子顯微技微加上倍頻訊號及一些屬於物理學上的光學特性，如光譜分佈等，即可大大的彌補了定量上缺乏。現階段生物體各機制的研究與發展一日千里，無論是分子生物或是生醫工程都大有展獲，但大部分都只在非活體之中的探索的階段，當然屬於生物體的事應當在生物體中得到解答。近年來，活體觀察成為最熱門的研究之一，其中chamber的使用，有效地增進了動物樣本及人體的研究，dorsal skinfold chamber為活體觀察實驗提供了一個更有效的管道。

關鍵字

非線性光學；雙光子；二倍頻；肺癌診斷；活體觀察

並列摘要

Lung cancer is the number one on the list of ten leading causes of death, for Chinese in the past few decades. To eliminate lung cancer, the first step is to develop a fast and efficient method for early detection and treatment. The core of this study is to develop a reliable system for lung cancer diagnosis. Using data collected from in vivo observation was in combination cancer diagnosis database developed from in vitro observation; we are steps closer to understanding cell growth. Two-photon differs from conventional microscopy in its capability to reinforce images from different depths. Two-photon fluorescence microscopy does not require lengthy histological sample preparation, is to obtain high quality optical images. The time required for complete diagnosis is therefore shorten with two-photon fluorescence microscopy. Furthermore, traditional diagnostic methods emphasize non-quantitative morphological differences. This shortcoming can be compensated by using two-photon fluorescence microscopy in conjunction with spectral analysis and second harmonic generation. Current research and development in the field of molecular biology and bio-medical engineering are progressing rapidly. Most of them are however in vitro experiments. It is more logical to answer questions regarding biological mechanisms through in vivo experiments. During the past few years, observation in vivo has become one of the most active researching fields. The invention need more description is instrumental to researches involving animals and humans. Dorsal skinfold chamber provides an efficient tool for observation in vivo. We developed an improved version of the chamber and used it to obtain images with higher resolutions and for extended period of in vivo observations.

並列關鍵字

non-liner ； two photon ； second harmonical generation ； lung cancer diagonosis ； in vivo research

參考文獻

[1] Dnaiel C Fernandez., Infrared spectroscopic imaging for histopathologic recognition. Nature Biotechonlogy. 23:469-474.(2005)

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[3] Alberto Diaspro, “Confocal and two-photon microscopy “ Wiley-Liss

[5] Sadison JC: The transparent chamber of the rabbit’s ear giving a complete description of improved techniques of construction and introduction and general accout of growth and behavior of living cells and tissues as seen with the microscope. AM J Anat 1928,41:447-472

[6] Algire, G .H & Legallais, F. Y. J. Natl. Cancer Inst. 10, 225-253 (1949)

國際替代計量

非線性光學顯微技術於肺癌診斷分析及活體研究平台之應用

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