探討微小核醣核酸-29b型於人類口腔鱗狀上皮細胞癌移行能力之影響

The Effect of miR-29b on Tumor Migration in Human Oral Squamous Cell Carcinoma

唐嘉御(Jia-Yu Tang)

指導教授 ： 張正琪

國立臺灣大學/牙醫專業學院/口腔生物科學研究所/碩士(2011年)

https://doi.org/10.6342/NTU.2011.10649

摘要

無資料

關鍵字

口腔鱗狀上皮細胞癌 ； 細胞移行 ； 微小核醣核酸 ； miR-29b ； CX3CL1

並列摘要

Purpose: We aim to examine target microRNA(s) interfering cell migration in established advanced migratory oral squamous cell carcinoma (OSCC) cell model, and correlate it with patient clinical status and survival. Underlying mechanism is also investigated.
Experimental design: microRNA profiling was performed in TW2.6 and advanced migratory cells, TW2.6 MS-10, which is selected from TW2.6. Target miRNA was quantitated with RT Q-PCR in 98 OSCC patients, and correlated to the pathological status. By bioinformatic PicTar analysis and IPA functional classification, downstream effector was indentified. Animal spontaneous experimental metastasis was evaluated by xenografting in SCID mice, and examined for lymph nodes metastasis.
Results: miR-29b is significantly increased in TW2.6 MS-10. Clinical data revealed that miR-29b expression related to lymph node metastasis and advanced tumor stage (P < 0.05, P < 0.05) in OSCC patients. Furthermore, multivariate analysis showed that miR-29b expression correlated to recurrence and indicated poor survival, which could be used as a prognosis tool. The in vitro study showed that miR-29b could promote OSCC cell migration ability (P < 0.05). miR-29b has been shown to down-regulate CX3CL1, a cell-cell adhesion regulator, which played the essential role in miR-29b-regulated OSCC　cell migration machinery. Additionally, CX3CL1 expression was shown to relate to lymph node metastasis and early tumor stage (P < 0.05, P < 0.05) and highly negatively correlate with miR-29b in OSCC patients (P < 0.05).
Conclusions: miR-29b, as an oncomir, promotes cell migration through CX3CL1 suppression from transcriptional level in OSCC. miR-29b provides a powerful tool for prognosis and potent therapeutic intervention.

並列關鍵字

oral squamous cell carcinoma ； migration ； microRNA ； miR-29b ； CX3CL1

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