自動化微流孔盤系統應用於稀少癌細胞藥物篩選之研究

Drug Screening of Single Cancer Cells in a SBS-based Microfluidic Plate

王聖毅(Sheng-Yi Wang)

指導教授 ： 胡文聰

國立臺灣大學/工學院/應用力學研究所/碩士(2013年)

https://doi.org/10.6342/NTU.2013.02130

摘要

在不少的疾病研究當中，稀少細胞顯現出具有相當的臨床價值，基於此本研究發展出一針對稀少細胞進行藥物測試之微流道設計。由於此微流孔盤中的獨特結構，在稀少的細胞數之下，例如病人檢體中的循環癌細胞(circulating tumor cell, CTC)，其數量相當稀少，在進行液體交換時此獨特的結構設計可確保細胞的保存率。另外此微流孔盤最突出的優勢為每個孔洞僅需要十顆左右的細胞以及大約9微升的試劑量便可進行實驗。此外，因為擁有標準化的設計，在液體交換方面可使用市面上的自動化液體交換機台進行。在完成藥物測試之後，利用染色的方式來分辨活細胞與死細胞，進而推算出細胞生存率。結果顯示利用傳統的96孔盤以及本研究所設計之微流孔盤所得道之藥物測試結果，在統計上沒有顯著的差異；另外，使用微流孔盤進行不同細胞數量之藥物測試之結果，兩者之間亦無顯著的差異。由此可證明利用本研究所設計之微流孔盤有能力進行稀少細胞藥物篩選。

關鍵字

藥物篩選 ； 微流體 ； 稀少細胞 ； 標準化設計 ； 自動化

並列摘要

Rare cells have been shown of high clinical significance in many disease scenarios. This work presents a microfluidic device for rare cell-based drug screening. With rare cell count, such as circulating tumor cell (CTC), from patient sample extremely low, unique features in the MicroGap Plate (MGP) enables high cell conservation during solution exchange. The most prominent feature of the MGP is that minimum of only about ten cells per well are needed in reagent volume of about 9μL. Moreover, the SBS standard design allows the use of automatic liquid handling work station in solution exchange. After drug treatment, cell viability was quantified by staining method to distinguish live cells and dead cells. Results show no statistical significance was found in dose-response between the MGP and the 96-well plate controls. Furthermore, the drug-responses between different cell counts on MGP have no statistical significance, which suggests that the MGP is capable of drug screening of rare cells.

並列關鍵字

Drug screening ； microfluidic ； rare cells ； standard design ； automation

參考文獻

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