番茄ERF36與ERF39在逆境反應之功能研究

Functional study of tomato ERF36 and ERF39 in stress responses

張芯(Sim Chong)

指導教授 ： 鄭秋萍

國立臺灣大學/生命科學院/植物科學研究所/碩士(2013年)

https://doi.org/10.6342/NTU.2013.10886

摘要

植物在自然環境中常遭受各種逆境脅迫，造成植物生長與農作物產量影響極巨，例如，由青枯病菌(Ralstonia solanacearum, Rs)引起的青枯病（bacterial wilt, BW）與缺水逆境都會導致植物急速死亡，造成農業上重大損失。植物為了生存演化出一套複雜的調控機制以調控並抵禦逆境反應，而乙烯轉錄因子（ethylene-response factor, ERFs）是植物特有的轉錄因子且參與許多逆境反應。我們先前的研究顯示在番茄中的兩個屬於VIII群ERF的成員SlERF36與SlERF39可能在青枯病與乾旱防禦反應具一定的重要性，故本研究旨在利用過量表現與基因靜默的轉殖植物研究策略進一步分析這兩個基因在植物逆境反應之確切功能與其調控機制。由結果發現SlERF36蛋白確實具有抑制轉錄活性的功能；過量表現SlERF36可能會使轉殖菸草對青枯病感病些微增加、對滲透壓逆境及乾旱抗性降低，推測SlERF36可能抑制抗病與抗旱相關啟動基因（activators）表現而造成這些性狀。另外，研究結果顯示SlERF39具有抑制轉錄活性的功能；過量表現SlERF39對青枯病抗性降低、對滲透壓及乾旱逆境反應降低，而較抗鹽害逆境，推論SlERF39可能使ET與JA訊息傳遞之抑制基因（repressors）表現降低進而增加ET與JA表現。SlERF39可能抑制促進基因表現進而對缺水逆境抗性降低，而藉由抑制啟動基因表現對鹽害逆境抗性增加。未來可利用本研究所得之過量表現與基因靜默之轉殖植物，更深入探討SlERF36與SlERF39在各種逆境反應之功能，希望有助於釐清對植物病害與非生物逆境之複雜的交互作用，並增加對這些逆境防禦機制的了解並加以運用。

關鍵字

乙烯反應轉錄因子 ； 青枯病 ； 乾旱逆境 ； 鹽害逆境

並列摘要

Plant constantly encounters environmental stresses such as drought, salt, heat even pathogens and pest, which effect plant growth and crop productivity. Bacterial wilt (BW), which is caused by Ralstonia solanacearum, is a very complex deadly disease and shares certain common features with water stress responses induced plant death and crop loss. Hence, the plants have evoluted a complex mechanism to regulate and defense to stress responses. Ethylene response factors (ERFs) is a large family of plant-specific transcription factors involved in various stress responses. Our previous study revealed that two Group VIII ERFs from tomato, namely SlERF36 and SlERF39 may involve in BW and drought responses. In this study, transgenic plant with altered expression levels of these genes were generated and analyzed for their stress responses and the involvement regulatory mechanism. The result show that SlERF36 was a functional transcriptional repressor. Overexpression of SlERF36 in transgenic tobacco plants demonstrated that SlERF36 increased susceptibility to BW, decreased osmotic and water tolerance. It is hypothesized that SlERF36 may reduce the expression of defense response and water tolerance response by suppressing the expression of defense response and drought tolerant activators. SlERF35 also function as a transcriptional repressor. Overexpression of SlERF39 cause increased susceptibility to BW and decreased in water stress tolerance, increased expression of ethylene (ET) and jasmonic acid (JA) biosynthesis genes. It is hypothesized that SlERF39 re-depressed the ET and JA signaling by suppressing the expression of certain repressors. Reduced expression of SA led to susceptibility to BW. SlERF39 may reduce the expression of water tolerance response by suppressing the expression of drought tolerant activators but increased the expression of salt tolerant through suppression of salt tolerant suppressor. The transgenic plants generated in this study can be used for future analyses to gain further insight into disease resistant and abiotic cross-talk mechanism of these proteins.

並列關鍵字

Ethylene-response factors (ERFs) ； bacteria wilt (BW) ； drought ； salt stress

參考文獻

楊宗霖。2011。ERF B1-a 基因群在植物青枯病與非生物逆境反應之功能研究。國立台灣大學植物科學研究所碩士論文。

Aguilar, I., Alamillo, J.M., Garcia-Olmedo, F., and Rodriguez-Palenzuela, P. (2002). Natural variability in the Arabidopsis response to infection with Erwinia carotovora subsp. carotovora. Planta 215, 205-209.

Asselbergh, B., Curvers, K., Franca, S.C., Audenaert, K., Vuylsteke, M., Van Breusegem, F., and Hofte, M. (2007). Resistance to Botrytis cinerea in sitiens, an abscisic acid-deficient tomato mutant, involves timely production of hydrogen peroxide and cell wall modifications in the epidermis. Plant Physiol 144, 1863-1877.

Atkinson, N.J., and Urwin, P.E. (2012). The interaction of plant biotic and abiotic stresses: from genes to the field. J Exp Bot 63, 3523-3543.

Ben, C., Debelle, F., Berges, H., Bellec, A., Jardinaud, M.F., Anson, P., Huguet, T., Gentzbittel, L., and Vailleau, F. (2013). MtQRRS1, an R-locus required for Medicago truncatula quantitative resistance to Ralstonia solanacearum. New Phytol 199, 758-772.