電荷可調節之膠體粒子於凝膠電泳之電動力學行為

Electric Kinetic Behavior of a Charge-regulated Particle in Gel Electrophoresis

黃治華(Chih-Hua Huang)

指導教授 ： 徐治平

國立臺灣大學/工學院/化學工程學研究所/博士(2014年)

https://doi.org/10.6342/NTU.2014.02354

摘要

凝膠電泳在近年來應用上逐漸廣泛被使用，甚至更勝於傳統一般電泳，主要因為凝膠孔隙所產生的流體黏滯效應及立體空間的屏蔽效應造成通過的待測樣本移動速度大幅降低而造成在檢定及觀測上提升其觀測之簡易度及準確性。因此，本論文主要以有限元素法探討膠體粒子在凝膠電泳模擬與實際實驗中所產生的差異性，如介電係數由於加入凝膠濃度的多寡而產生改變等等。另外也同時去探討表面可解離性質之粒子在凝膠電泳中之電泳行為以區分與一般電泳之差異性及優越處，如一般電泳所產生之電雙層極化效應於高凝膠濃度下減低其效應以及表面可解離性質之粒子於不同濃度或酸鹼值下產生的不同電泳行為。最後更配合實驗趨勢之要求，考慮電泳本身所產生的焦耳效應以及為了實驗設計所改變的不同高低環境溫度，討論於不同的溫度下，表面可解離粒子所展現出不同特質之電泳行為。本篇論文主要指出加入凝膠濃度改變溶液介電係數且同時影響極化效應之發生強弱與否，進而模擬出表面可解離粒子三階段電泳行為，最後改變溫度歸納出表面可解離粒子於不同溫度下展現之各種電動力學行為。

關鍵字

凝膠電泳 ； 介電係數 ； 表面可解離 ； 電雙層極化效應 ； 焦耳效應 ； 溫度效應

並列摘要

Gel electrophoresis is adopted gradually and widely in all kinds of biochemistry experiments in recent decades and even more than conventional electrophoresis because the hydrodynamic effect arising from the liquid drag and the steric effect from particle-polymer interaction reduce the mobility of sample particles to make the observation easier and enhance the degree of accuracy. Besides in this study, we apply the finite element method to simulate gel electrophoresis and try to investigate the difference between simulations and real experiments, e.g., the changing of dielectric constant caused by the addition of gel. Then we still consider the particle property as charge-regulated for the similarity of real experiments and analyze the results of simulation to understand the difference and the advantage of gel electrophoresis, for instance, high gel weight percentage causes the weakness of the electric double layer polarization and various pH value or electrolyte (buffer solution) concentration affects the variation of the electric kinetic behavior. Eventually we match up the trend of real experiments and consider the Joule effect caused by applied electric field and the temperature effect to design different environmental temperature. Then the analysis of electric kinetic behavior of a charge-regulated under various temperatures also includes in this study. The whole thesis mainly indicates that the dielectric constant of the solution decreases by increasing the gel concentration then also affects if electric double layer polarization appears. Furthermore we also analyze the three various stages of electric kinetic behavior of a charge-regulated particle and eventually investigate how the temperature effect to affect a charge-regulated particle to emerge all various kinds of electric kinetic behaviors.

並列關鍵字

gel electrophoresis ； dielectric constant ； charge-regulated ； electric double layer polarization ； Joule effect ； temperature effect

參考文獻

Chapter 1

[5] Stiger, D., Macromolecules 2000, 33, 8878.

[13] Brinkman, H. C., Appl. Sci. Res. 1947, A1, 27.

[14] Debye, P., Bueche, A. M., J. Chem. Phys. 1948, 16, 573.

[15] Brady, J. F., Hindered Diffusion, American Institute of Chemical Engineers, AnnualMeeting, San Francisco, CA 1994, November 13.