蝦白點症病毒極晚期基因之篩選

Transcript screening for very late genes of white spot syndrome virus(WSSV)

易德明(Der-Ming Yi)

指導教授 ： 羅竹芳 ； 郭光雄

國立臺灣大學/生命科學院/動物學研究研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.10199

摘要

由白點症病毒微陣列實驗結果得到此病毒523個開放譯讀區(open reading frames, orfs)的基因初步轉錄資料，並且從中篩選出13個於感染後36小時才開始轉錄的開放譯讀區。經由反轉錄酶-聚合酶鏈反應的時序分析結果確認了其中8個orfs可能為白點症病毒極晚期基因候選。透過極晚期基因候選的胺基酸序列之比對與分析，發現了三種不同區域所代表的弁鄍i能與病毒感染晚期所表現出的現象有關。白點症病毒極晚期基因候選的發現提供此病毒另一項新的研究領域，而經由極晚期基因候選的弁鄐尷R將能更瞭解有關此病毒與宿主之間的互動關係。

關鍵字

微陣列 ； 極晚期基因 ； 蝦白點症病毒

並列摘要

Microarray of time-course white spot syndrome virus (WSSV)-infected Penaeus monodon presents a transcription profile. The result reveals 13 open reading frames(orfs) that transcribed at approximately 36 hours post infection. Temporal analysis of 13 orfs by RT-PCR confirms the result of WSSV microarray and reveals that 8 orfs may be the very late gene candidates of WSSV. Motif and domain prediction results of WSSV very late gene candidates significantly discovered three types of regions that may contribute to the phenomena of WSSV infection very late stage. WSSV very late gene may function as anti-apoptosis genes or host immune evasions, which were advantageous in virion replication. Discovery of WSSV very late gene candidates provide a novel field of WSSV genomic research. In addition, functional analysis of WSSV very late gene candidates gives more implications about biological interaction between WSSV and its hosts. WSSV very late gene candidates may be crucial factors for providing solutions for the epidemic of WSSV.

並列關鍵字

microarray ； very late genes ； white spot syndrome virus

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