應用螢光顯微術比較分析兩肺腺癌細胞株CL1-0及CL1-5細胞核染色質

Comparative analysis of nucleus chromosome of lung adenocarcinoma cell lines, CL1-0 and CL1-5, by fluorescence microscopy

傅學聖(Xue-Sheng Fu)

指導教授 ： 董成淵

國立臺灣大學/理學院/物理學研究所/碩士(2016年)

https://doi.org/10.6342/NTU201601195

摘要

在所有癌症中肺癌是最致命的一種，近幾年在臺灣肺癌時常居於癌症十大死因之首。為了降低肺癌的死亡率，在肺癌早期時發現能幫助病人即時的得到適當的治療。在腫瘤細胞中的DNA含量異常是一個被觀察到的特徵，有些研究顯示這有用來預測病情的潛力。在本論文中為了找出轉移能力和DNA含量異常的關係，使用了兩種不同轉移能力的肺腺癌細胞株CL1-0及CL1-5並使用DAPI這種染劑對細胞核染色。透過螢光顯微術，將DNA含量以螢光訊號量化，並得到細胞核DNA含量的分布圖。從這些DNA含量的資料發現，DNA含量異常越顯著的細胞株，其轉移能力亦越強。此外，計算一群細胞的DNA含量的標準差，有做為轉移能力指標的潛力。

關鍵字

肺腺癌 ； CL1-0 ； CL1-5 ； 雙光子顯微術 ； DAPI ； 細胞核 ； DNA含量異常

並列摘要

Lung cancer is the most lethal cancer. In Taiwan, the mortality rate of lung cancer ranks first among the cancer-related mortality rate in recent years. To reduce the morality, the early detection of the lung cancer may help the patient get the proper treatment in time. The DNA content abnormality is one of the characteristics which are observed in the tumor cells. Some researches show the potential prognostic value of it. In this thesis, to find the relation between invasive ability and DNA content abnormality, we use the two lung adenocarcinoma cell lines, CL1-0 and CL-5 which have different invasive ability and stain their nuclei with DAPI. By the fluorescence microscopy technique, quantify the DNA content of nuclei with the fluorescence and get nuclei DNA content distribution histogram. From the nuclei DNA content data, we find that the cell line with higher invasive ability has larger DNA content abnormality and the standard deviation of the DNA content of the cells may have potential to be the invasive ability index.

並列關鍵字

lung adenocarcinoma ； CL1-0 ； CL1-5 ； two-photon microscopy ； DAPI ； nucleus ； DNA content abnormality

參考文獻

[1] 國家衛生福利部, “民國103年主要死因分析,” (2016)

[2] Siegel, R., et al. (2013). "Cancer statistics, 2013." CA: A Cancer Journal for Clinicians 63(1): 11-30.

[3] American Cancer Society

[4] Team, T. N. L. S. T. R. (2011). "Reduced Lung-Cancer Mortality with Low-Dose Computed Tomographic Screening." New England Journal of Medicine 365(5): 395-409.

[5] Dyszkiewicz, W., et al. (2000). "Prognostic significance of DNA ploidy in squamous cell lung carcinoma: is it really worth it?" The Annals of Thoracic Surgery 70(5): 1629-1633.