快速脈衝式量測之微電泳分析

電泳為長久以來生物醫學界用以分離不同帶電量�分子量比例之生物分子片段，並作為診斷依據的技術。但傳統電泳長期存在一些無法突破之缺點，其中有大部分可由電子工程學的角度去切入分析將生物分子之電泳，在電學觀念上作出革新。首先，傳統電泳中使用之緩衝介質在外加高電場下，由於焦耳熱造成熱擴散、對流及擾動等效應，會降低生物分子分離效率，故傳統電泳無法外加高電場作分析；且其分析耗時、不易得到重複性。亦增加實驗操作人員中毒與致癌等風險。故日後我們預訂朝向新型微流道電泳發展。本篇論文提供一種以半導體物理觀念來分析解釋DNA在外加脈衝電場下的運動行為。只需在DNA 溶液中，外加一高頻電壓脈衝，即可快速得到不同分子量的 DNA 組成；而不必像傳統電泳般實際進行物質分離。藉此時變電場觀察不同種類、分子量、濃度的DNA 之「電阻－電容（RC）響應」特性，期望能在相同外加條件之下得到可重複的響應曲線；並引入半導體物理之雙極擴散方程式來分析 DNA 在外加脈衝電場下的運動模式。並且在多次量測之後，得到一個電流－電壓（I-V）曲線，從此曲線中分析在不同外加偏壓下的DNA電流成分，以及不同分子量 DNA電流大小之成因。初步已確定此種新分析方式的可行性及可重複性，期待在日後累積更多統計資料之後，以半導體物理的理論模型來分析生物分子產生的快速物理機制，進而為生物醫學界的診斷分析鑑定技術，帶來重大的突破。

關鍵字

脈衝式量測；電泳；微流道元件

並列摘要

Electrophoresis is a technique has long been used to separate biomolecule fragments with different e/m ratios in accordance with medical diagnosis. But there were some shortcomings have long been existed but still cannot be overcome in traditional electrophoresis. Most of which could be analyzed and be taken innovation in a point of view of nanoelectronic technology. First, the buffer medium used in traditional electrophoresis leads to decrease the biomolecular separation efficiency because of thermal diffusion, convection and perturbation motion. That is why high applied electric field could not suitable for old type electrophoresis. Besides, we also want to exclude some poor properties like time-consuming, low repeatability, and use of dangerous carcinogenic fluorescent dye, etc. In this thesis, we bring up a concept base on semiconductor physics to analysis the movement of DNA macroions under an applied impulse voltage. We can obtain the DNA composition with different molecular weights rapidly with just feeding an impulse with high-frequency instead of really “separating” them. By observing RC transient response of different bases, molecular weights and concentrations, we expect that the response curve under the same measuring condition can be repeated. Then we import Ambipolar equations in semiconductor physics to elucidate DNA movement and distribution under applied impulses. And we got an I-V characteristic after sufficient times for measuring, analyzing current component and the reasons of current difference among different DNA sequences. We preliminary confirmed the novel analysis technique is practicable and repeatable. After sufficient data are being acquired, we decide to establish a theoretical model to analyze biomolecular rapid mechanism, and then making a breakthrough for biomedical diagnosis and identification.

並列關鍵字

impulse measurement ； electrophoresis ； microfluidic device

參考文獻

[1] Matthews, Christopher K., KE Van Holde and Kevin G.Ahern. Biochemistry. 3rd ed. Menlo Park, CA:Benjamin Cummings Publishing Company, pp 52-56(1996)

[2] D. J. Beebe, et al., “Ultra rapid prototyping of microfluidic systems using liquid phase photopolymerization”, Lab-on-a-Chip, 2 (l), pp50-55(2002)

[3] Jacobson S C, Hergenroder R, Koutny L B, Warmack R J and Ramsey J M. Effects of injection schemes and column geometry on the performance of microchip electrophoresis devices, Anal. Chem. 66 1107–13(1994)

[4] Schmalzing D, Koutny L, Adourian A, Belgrader P, Matsudaira P and Ehrlich D. DNA typing in thirty seconds with a microfabricated device Proc. Natl Acad. Sci. USA 94 10273–8(1997)

[5] Ajdari, A., Appl. Phys. A(75), 271–274(2002)

國際替代計量

快速脈衝式量測之微電泳分析

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