甘藷中鈣離子與一氧化碳對於傷害誘導基因ipomoelin表現的影響

Effect of calcium ion and carbon monoxide on the expression of wound-inducible ipomoelin gene from sweet potato

林信宏(Hsin-Hung Lin)

指導教授 ： 鄭石通

國立臺灣大學/生命科學院/植物科學研究所/博士(2009年)

https://doi.org/10.6342/NTU.2009.10546

摘要

無資料

關鍵字

甘藷 ； 一氧化碳 ； 鈣離子 ； 傷害誘導基因

並列摘要

The ipomoelin gene (IPO) was identified to be a wound inducible gene from sweet potato (Ipomoea batatas cv Tainung 57), and its expression was stimulated by ethylene, methyl jasmonate and hydrogen peroxide. IPO protein was also characterized as a defense related protein and it is a carbohydrate-binding protein. Recently, carbon monoxide (CO) was reported to play an important role in stress response and development, such as oxidative stress, salt stress, and root formation, in plants. The role of CO involved in wounding response has been further studied here. In the present of CO, the wound-induced IPO expression was inhibited. Heme oxygenase, which is a CO producing enzyme, was regulated in sweet potato to control IPO expression. CO induced the phosphorylation of p38-like MAPK in sweet potato, and negatively regulated the wound-induced IPO expression. Furthermore, the increase of cytosolic Ca2+ and the production of hydrogen peroxide were previously reported to be the main signal transducer inducing IPO expression. We found CO inhibited the accumulation of cytosolic Ca2+ stimulated by mechanical wounding. Besides, the production of hydrogen peroxide induced by wounding was also inhibited by catalase induced by CO. Finally, IPO gene is activated by dephosphorylated protein(s) via protein phosphatase, and that the position of dephosphorylated protein(s) is located in the very downstream of the signal transduction pathway for IPO expression.

並列關鍵字

calcium ion ； carbon monoxide ； wound-inducible gene ； ipomoelin ； sweet potato

參考文獻

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