- 學位論文
巴西洋菇食療方對四氯化碳誘導大鼠氧化性肝損傷之護肝功效評估
Evaluation of hepatic protection of Agaricus blazei-based herbal extract against CCl4-induced oxidative liver injury
摘要
本研究為利用衛生署公告之護肝功能動物模式，探討味全公司研發之養甘露樣品，是否具有對於四氯化碳誘導大鼠肝損傷保護作用。本實驗分為六組，分別為正常控制組 (未經四氯化碳處理)、四氯化碳處理負對照組 (僅投予四氯化碳之處理組)、Silymarin處理正對照組 (四氯化碳+Silymarin處理組) 及1X、2X、5X劑量實驗組 (四氯化碳+740、1480、3700 mg/kg bw/day養甘露樣品處理組)。實驗結果顯示，經連續餵食八週養甘露樣品後，三種劑量之養甘露樣品皆可以增加肝臟中抗氧化物質glutathione (GSH) 的含量並且使大鼠肝臟中脂質過氧化情況改善以及肝臟生化功能指標酵素丙胺酸轉胺酶 (alanine aminotransferase, ALT)、天門冬胺酸轉胺酶 (aspartate aminotransferase, AST) 數值顯著的減少，表示應具有護肝之功能 (p < 0.05)。在肝臟抗氧化及解毒代謝酵素活性方面，2X、5X劑量養甘露樣品處理組之麩胱甘肽過氧化酶 (glutathione peroxidase, GPx1)、麩胱甘肽還原酶 (glutathione reductase, GRd)、麩胱甘肽硫轉移酶 (glutathione S-transferase, GST)、超氧化物歧化酶 (superoxide dismutase, SOD) 酵素活性均顯著高於四氯化碳處理負對照組 (p < 0.05)。在四氯化碳投予後之第八週血清中三酸甘油酯 (triglyceride, TG) 值方面，三種劑量養甘露樣品處理實驗組之TG均較四氯化碳處理負對照組低且達顯著性差異 (p < 0.05)。此外，肝臟組織病理學觀察方面，5X劑量養甘露樣品可使肝細胞發炎壞死、脂肪變性及肝臟纖維化等現象都有顯著的改善 (p < 0.05)。由以上實驗結果推測養甘露樣品對於保護肝臟應具有正面的效果。
並列摘要
Mushrooms have been used as an important source of nutrition and/or therapy throughout the world since ancient times. Agaricus blazei polysaccharides has been reported to induced with immunomodulatory and antitumor activities. This study was designed to evaluate the effects of Agaricus blazei-based herbal extract on CCl4-induced chronic hepatotoxicity in rats. Male Wistar rats (210 ±10 g) were divided into six groups in this study, including normal control (not treated with CCl4) and groups treated with CCl4, i.e. negative control (only treated with CCl4), positive control (treated with CCl4 and Silymarin), and three experimental groups (treated with CCl4 and low, medium or high dose of Agaricus blazei-based herbal extract, i.e. 0.74, 1.48, or 3.7g/kg bw/day Agaricus blazei-based herbal extract, respectively). On the other hand, rats were administrated with Agaricus blazei-based herbal extract for one week before the administration of CCl4. Rats were treated with Agaricus blazei-based herbal extract once a day until the eighth week of CCl4 treatment. Blood samples were collected for assaying serum biological parameters. The livers were excised for evaluating peroxidation products and antioxidant substances, as well as the activities of antioxidant enzymes. Pathological histology was also performed. The results showed that the Agaricus blazei-based herbal extract significantly (p<0.05) lowered the serum levels of hepatic enzyme markers (alanine aminotransferase, aspartate aminotransferase), inhibited inflammation, necrosis, fatty degeneration and fibrosis. Also reduced the content of the peroxidation product malondialdehyde, and the elevated the content of reduced form of glutathione (GSH). Treatment of rats with Agaricus blazei-based herbal extract could increase the activities of glutathione peroxidase (GPx1), glutathione reductase (GRd) and glutathione S-transferase (GST) in liver when compared with CCl4-treated group (p<0.05). Therefore, the results of this study show that Agaricus blazei-based herbal extract can be proposed to protect the liver against CCl4-induced oxidative damage in rats, and the hepatoprotective effect might be correlated with its antioxidant and free radical scavenger effects.