細菌發酵產物調節吞噬細胞之吞噬及活性氧化物產生之能力

Modulation of Phagocytic Cell Phaocytosis and Reactive Oxygen Species Production by Bacteria Fermentation Product

林韋君(Wei-Chung Lin)

指導教授 ： 賈景山

國立臺灣大學/醫學院/微生物學研究所/碩士(2008年)

https://doi.org/10.6342/NTU.2008.01619

摘要

吞噬作用是當感染原進入宿主體內時，最先引起的先天性免疫反應，而嗜中性球及巨噬細胞則扮演了主要吞噬感染原的角色。之前許多研究指出，細菌發酵產物具有免疫調節功能，且在服用乳酸菌的發酵產物後，受測者周邊血液中單核球的吞噬能力，有明顯上升的現象。在本研究中，我們建立體外的免疫細胞吞噬作用模式，測試豆類細菌發酵產物是否具有直接增強細胞吞噬能力。先以4%無菌硫乙醇酸鹽由腹腔注射老鼠，隔天分離出腹水細胞，包括嗜中性球及巨噬細胞，以細菌發酵產物刺激不同時間點後，加入帶有綠色螢光蛋白的大腸桿菌，利用流式細胞儀分析腹水細胞的吞噬能力，並利用H DCFA檢測細胞內活性氧的產生量。結果顯示細菌發酵產物分子量大於50 kDa的部分，具有促進腹水吞噬細胞及人類單核球細胞株THP-1吞噬作用的效果，此外，分子量小於50 kDa的細菌發酵產物抑制脂多醣體或大腸桿菌所引起小鼠腹水吞噬細胞及人類單核球細胞株的活性氧化物產生。本實驗結果顯示，細菌發酵產物具多重活性，除了可促進吞噬細胞的功能外，也可調節氧化壓力的過度產生。

關鍵字

大豆發酵產物 ； 吞噬細胞 ； 吞噬作用 ； 活性氧分子 ； 抗氧化物

並列摘要

Phagocytosis is the first line of host defense against pathogens and is mediated by phagocytes including macrophages and neutrophils. The foods fermented products of lactic acid bacteria have the capacity to modulate immune function. In animals feeding with lactic acid bacteria fermented products, the phagocytic activity of peripheral blood mononuclear cells could be enhanced. In this study, we established an in vitro phagocytosis assay to investigate whether a soybean fermented has a direct phagocytosis-stimulating effects. After intra-peritoneal injected with 4% thioglycollate overnight, Balb/c mice were sacrificed and the peritoneal cells, neutrophils and macrophages were collected. Following stimulation with soybean fermented product, these phagocytic cells were challenged with GFP-expressed E. coli. Phagocytosis was semi-quantitated by flow cytometry at different time intervals. In parallel experiments, ROS production by primary phagocytes or cell lines was measured by H DCFA. The fermented products or its fractions harboring componnts greater than 50 kDa could enhance the phagocytic ability of mouse phagocytes or human macrophage cell line, THP-1. On the other hands, the fractions less than 50 kDa could inhibit LPS- or bacteria-induced ROS production in mouse phagocytes or THP-1. The results indicated that the soybean fermented product exhibit complex activities from enhancement of the phagocytosis to the regulation of oxidative stress in phagocytic cells.

並列關鍵字

Soybean fermentation product ； phagocytic cells ； phagocytosis ； ROS ； antioxidant

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