紅外線照射對神經細胞表現影響之研究

The study of infrared radiation effect on neurons

劉智揚(Chih-Yang Liu)

指導教授 ： 林致廷

國立臺灣大學/電機資訊學院/電子工程學研究所/碩士(2014年)

https://doi.org/10.6342/NTU.2014.11079

摘要

近年來紅外線光在各方面的研究及應用均非常熱門，對植物體及動物體，尤其是人體的影響，更是深感興趣，又其中神經系統是動物體最重要的聯絡和控制系統，它能指示身體做出適當的反應，來保護自己和生存，因此如何治療及修復受損的神經組織為一大重要課題。而研究人員使用各種實驗方法欲了解紅外線光對神經系統的變化，但目前對其主要的原理或機制了解的並不多，所以此領域依舊有許多謎團需要挖掘。

本研究的目的，是希望透過紅外線光照射老鼠腎上腺髓質副神經節腫瘤細胞(PC12)及中腦神經細胞的實驗，來了解紅外光照射對神經細胞可能產生的影響。本實驗中設計一種整合紅外線發射器和二氧化碳培養箱的設備，借此模擬紅外光照射老鼠神經細胞，並觀察細胞生長情形及蛋白質表現。在我們之前的研究發現，將PC12細胞經過波長2∼4.5um之紅外光照射及培養4天後，其覆蓋率及某些蛋白質表現會有變化。接下來的研究，我們首先用西方墨點法(Western Blotting)分析照光後PC12細胞中肌動蛋白(Actin)及微管蛋白(Tubulin)的表現量，發現肌動蛋白和微管蛋白表現量具有差異。接著再用老鼠的中腦神經細胞，觀察照光後其生長狀態及複雜度，實驗顯示神經細胞在紅外光的照射下會導致其複雜度的改變。最後為了瞭解紅外光如何影響神經細胞的生長及其機制，我們應用新一代定序(Next Generation Sequencing)分析神經細胞中miRNA的表現，藉此了解miRNA如何影響神經細胞成長或造成蛋白質變化的原因。

關鍵字

紅外線 ； 神經細胞 ； 西方墨點法 ； 新一代定序 ； 細胞骨架

並列摘要

In recent years, infrared light has been vastly popular and applied in many areas. It is more fascinating when it comes to the impact on animals、plants and especially human beings. Particularly speaking, nervous system is extremely important for animals to communicate 、control, which enables them to react properly according to the signal from the body, and even protect themselves. Therefore, how to cure and restore impaired nervous tissue becomes a critical issue. Researchers have used a variety of experiments to unveil the influence of infrared light on nervous system; however, it is little known about the principals and mechanisms. As a result, more mysteries should be resolved.

The purpose of the thesis is to figure out the potential impacts of infrared light on nervous cells by applying infrared light on PC12 and midbrain neuron. By designing an equipment combined with Infrared emitter and Carbon dioxide incubator to simulate the situation in which neurons lit by infrared light and observe cell growth and protein expression. From previous studies, cell coverage and some protein expression would be affected by applying 2∼4.5um infrared light on culture P12 cell for 4 days. For subsequent researches, there will be differences between the expression of Actin and Tubulin on PC12 by making use of Western Blotting Analysis. Moreover, utilizing midbrain neuron of rats to observe cell growth and complexity. The experiment reveals that the complexity will be changed while neurons are exposed to infrared light. Hence, in order to understand how neuron growth and the mechanism are being affected by infrared light, we take advantage of Next Generation Sequencing to analyze the miRNA expression in neurons; and, furthermore, figure out how cell growth or protein variations being influenced by miRNA.

並列關鍵字

Infrared ； Neurons ； Western Blot ； Deep Sequencing ； cytoskeleton

參考文獻

[1] Chen, C.F. , Effect on narrow bandwidth infrared radiation on mungbean growth and expression. NTU, 2007.

[2] Hu, S.W., Effect on narrow bandwidth infrared radiation on araiabidopsisthaliana growth and expression. NTU, 2009.

[3] HIDEYOSHI TOYOKAWA , et al., Promotive effects of far-infrared ray on full-thickness skin wound healing in rats . Exp Biol Med , 2003. 228(6): p.724-729.

[4] Wells, J., et al., Application of infrared light for in vivo neural stimulation , Journal of Biomedical Optics , 2005. 10(6): p. 064003-1.

[5] Ana P. Keef, Comparison Between Infrared and Electrical Nerve Stimulation. Engineering in Medicine and Biology Workshop, IEEE Dallas, 2007. p.114-117.