多功能性生醫奈米材料之合成與應用

Synthesis, Characterization and Bio-application of Multifunctional Nanomaterials

蕭怡瑄(Yi-Hsuan Hsiao)

指導教授 ： 周必泰

國立臺灣大學/理學院/化學研究所/碩士(2012年)

https://doi.org/10.6342/NTU.2012.10824

摘要

無資料

關鍵字

核磁共振顯影 ； 細胞影像 ； 光動力學療法 ； 介孔洞二氧化矽 ； 藥物傳遞

並列摘要

Nanotechnology has attracted numerous attentions as materials at this scale possess different properties compared to their bulk counterparts. In addition to synthesizing nanomaterials of different kinds, it is also important to analyze and explore the applications of these unique structures. Herein, we report the synthesis of different kinds of nanomaterials possessing the traits of MRI signal or fluorescence imaging, such as H-MnO@mSiO2(Ir)-FA, insulin–Au nanoclusters, and carbon dots, and we also study the bio-application of these unique structures. By utilizing the high resolution of confocal microscopy, magnetic resonance imaging, and other cellular assays, we can realize the feasibility and biocompatibility of these nanomaterials.
Chapter Ι reports for the first time the insulin-directed synthesis of fluorescent gold NDs. The as-prepared insulin-Au NDs show intense red fluorescence ( λem: 670 nm, Φf : 0.07), excellent biocompatibility and preservation of natural insulin bioactivity in lowering the blood-glucose level. The versatility in applications is demonstrated via fluorescence imaging, X-ray computed tomography, insulin-inhibitor interactions, and preservation of nature insulin bioactivity in lowering the blood-glucose level.
In Chapter Ⅱ, we report the development of multi-functional uniform nanoparticles comprising a hollow manganese oxide core and a functionalized mesoporous silica shell (H–MnO@mSiO2(Ir)–FA) for use as theranostic nanocomposites that combine four-in-one functionality. The H–MnO@mSiO2(Ir)–FA nanoparticles (NPs) demonstrate its versatility in various applications. (1) The magnetic core provides the capability for T1 magnetic resonance imaging. (2) Phosphorescent iridium complex serves as both photosensitizer and the luminescent chromophore. (3) The nano-channels in the mesoporous silica shell increase the free diffusion of oxygen and water permeability, enhancing photodynamic therapy (PDT) and T1 signal, respectively. (4) Folic acid anchored on the outermost surface is able to target at specific cells for bimodal imaging and PDT.
In Chapter Ⅲ, we report on a facile method to synthesize carbon dots (CDs) using glycerol solvent as a single precursor via a pyrolysis process free from catalysts. This method is extremely simple and economical, which provides a doable way for mass production of highly emissive CDs. Further systematic improvement of particles homogeneity is made by harnessing the growth of CDs inside the mesoporous silica nanoparticles that act as a nano-reactor to regulate the size distribution. The as prepared CDs@mSiO2–PEG nanocomposites are then loaded with anti-cancer drug doxorubicin (DOX), so that the controlled-release of DOX could be well monitored by both time-dependent and spatially resolved ratiometric fluorescence intensity for CDs versus DOX in HeLa cells, demonstrating the CDs@mSiO2–PEG nanocomposites suited for cell imaging and drug release.

並列關鍵字

MRI contrast agent ； cell imaging ； photodynamic therapy ； mesoporous silica ； drug release

參考文獻

Chapter Ι: Insulin-Directed Synthesis of Fluorescent Gold Nanoclusters: Preservation of Insulin Bioactivity and Versatility in Cell Imaging

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