This study design the sand column tests in the laboratory to evaluate permeability and efficiency of substrates by using permeation grouting technology. The results show, the coarser the test sand and the lower the substrates viscosity, the better the grouting effect. If more than 5% fines added will affect the grouting results significantly. Spearman’s rank correlation coefficient and stepwise regression method were applied to analysis 36 sets of sand columns testing results. The relationships among the effective size of test sand (D10), the viscosity of substrates (μ)and grouting height, grouting time, grouting rate and outflow rate were obtained. The results of this study might be helpful for applying the permeation grouting technology to remedy the contaminated groundwater in the field.