初乳之免疫調節作用及對人類白血病細胞(U937)之抑制效果

Colostrum’s Immunomodulatory Activities and Inhibitory Effects on Human Leukemic U937 Cells

宮華婷(Hua-Ting Kung)

指導教授 ： 吳瑞碧

國立臺灣大學/生物資源暨農學院/食品科技研究所/碩士(2006年)

https://doi.org/10.6342/NTU.2006.02131

摘要

初乳泛指哺乳類動物於生產後初期所分泌之乳汁。初乳與常乳在營養成分及免疫因子的組成上有很大的差別，例如初乳含有較高量之生長、免疫調節及抗菌因子等。嬰兒剛出生時，初乳能夠給予嬰兒適當的免疫防護，在幼體免疫系統發育成熟正常運作前，避免外在病原體之感染。本實驗採用第一天至第七天牛初乳，探討其對於人類周邊血液單核細胞生長之效果及經由間接模式對人類白血病細胞U937抑制之作用。結果發現，第一天與第二天初乳有促進人類單核細胞生長之能力，且在最佳濃度300 μg/mL下能有效的增加T細胞、Tc細胞與自然殺手細胞之比率。而在初乳對於人類白血病細胞U937生長抑制方面發現，唯有第一天到第四天初乳能有效的間接抑制U937細胞之生長，並誘導其分化為正常之人類單核球或巨噬細胞。另外，由初乳與單核細胞所共同培養之條件培養液中，亦可測得具有免疫調節作用之細胞激素IL-1β、IFN-γ及TNF-α。
乳鐵蛋白為初乳中最常被探討具有免疫活性之蛋白質，其在初乳中含量最高，由前人研究發現乳鐵蛋白含量隨著初乳天數增加而明顯降低。利用細胞實驗探討乳鐵蛋白在初乳免疫調節活性中所扮演的角色，實驗結果發現乳鐵蛋白能促進單核細胞的生長，但在間接抑制U937細胞生長方面並不明顯。此外，初乳以酸沉澱法初步分離的結果發現，含有乳鐵蛋白之沉澱物具有對於人類單核細胞生長之功效，而上清液則無效果。

關鍵字

初乳 ； 人類白血病細胞

並列摘要

Colostrum is breast milk produced after giving birth and lasts for 2-4 days. Bovine colostrum contains essential nutrients, growth factors, antimicrobial compounds and immune-regulating constituents either not present in milk or present in substantially lower concentrations. Colostrum is thought to protect neonates from infection, as well as to facilitate the immune maturation. In this study the immunomodulatory activities and anti-proliferation effect of human lukemic cell U937 were being invested. Results suggested that colostrum collected on day 1 and 2 after onset of lactation enhanced the growth of peripheral blood mononuclear cells (MNCs) in a dose dependent manner and significantly increased the population of T cell、Tc cell and NK cell. Moreover, day 1 to day 4 colostrums were capable to induce MNCs to secrete IL-1β, IFN-γ and TNF-α, which subsequently inhibited the growth of U937 cells and further induced it’s differentiation into mature monocytes or macrophages.
Lactoferrin is the most prominent immunomodulatory factor within colostrum. It has been proven that lactoferrin is at its maximum in the colostrum obtained at parturition and gradually decrease with time course of lactation. The present investigation demonstrated that lactoferrin, even at very low concentration, could enhance the growth of PBMC. However, its effect on growth inhibition of U937 was not significant. In addition, when colostrum was fractionated by acid precipitation, the MNC growth effect was found in the precipitated fraction containing lactoferrin.

並列關鍵字

Colostrum ； Human Leukemic U937 Cells

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