不同細胞培養在親水性及疏水性高分子薄膜上電泳行為之研究

摘要本實驗主要探討不同的細胞以體外培養的方式種植於不同的高分子薄膜上，利用細胞電泳實驗來觀察不同的細胞在親水性及疏水性高分子薄膜上貼附情形及高分子薄膜親水及疏水性質對細胞膜表面電位之影響。從實驗的結果我們發現，在親水性聚乙烯醇（PVA）高分子薄膜上，細胞的貼附情形並不好。而在疏水性聚偏二氟乙烯（PVDF）或是組織培養聚苯乙烯（TCPS）上的貼附情形較好。而從細胞電泳的實驗中我們發現，培養的基材不同，其細胞的電泳運動性亦不同。亦即培養基材會造成細胞膜表面的電位不同，因而反應在其電泳運動性上。由實驗的結果顯示，當細胞種植於親水性基材PVA上一小時後，其運動性較高，亦即細胞種植於親水性基材時，其細胞表面帶電量較多。若將細胞種植於疏水性基材PVDF上，則其運動性相對的較低。即細胞培養於疏水性基材時，其表面帶電量較少。另外對同一種基材而言，貼附於基材上的細胞，其電泳運動性較未貼附於基材上者高。顯示細胞貼附於基材上時，其表面帶電量較多。另外藉由細胞電泳實驗亦可探討細胞與培養基材之間作用力的關係。關鍵字：聚乙烯醇、聚偏二氟乙烯、細胞電泳

關鍵字

聚乙烯醇；聚偏二氟乙烯；細胞電泳

並列摘要

ABSTRACT In this study, various cells were cultured on different polymer membranes in vitro. We investigated the adhesion of different cells on hydrophilic and hydrophobic polymer membranes and the influence of that on the surface charges of the plasma membranes by microelectrophoresis technique. The results suggested that cell adhesion on the hydrophilic PVA membrane was not very well, but those on the hydrophobic PVDF or TCPS membrane were better. From the experiment of microelectrophoresis, cells cultured on different substrates have dissimilar electrophoretic mobility. This result indicates that the substrates will affect the surface charges of the plasma membranes and respondes to electrophoretic mobility. When cells were cultured on hydrophilic PVA substrate for one hour, the electrophoretic mobility was higher than that on the hydrophobic PVDF substrate. Moreover, the electrophoetic mobility of the cells adhered on substrate were higher than that of those cells suspended on substrate. Keyword：microelectrophoresis, PVA, PVDF, hydrophilic,hydrophobic

並列關鍵字

microelectrophoresis ； PVA ； PVDF ； hydrophilic ； hydrophobic

參考文獻

1. Haydon,D.A.,Seaman,G.V.“Electrokinetic studies on the ultrastructure of human erythrocyte Ι.Electrophoresis at high ionic strengh-the cell as a polyanion”, Arch Biochem Biophys.,122, 126-136,1967

2. Webb,K., Hlady,V.,Tresco,P.A.,“Relationships among cell attachment,spreading,cytoskeletal organization, and migration rate for anchorage-dependent cells on model surfaces”.J Biomed Mater Res.,49, 362-368,2000

3. Krasteva,N.,Harms,N.,Albrecht,W.,Seifert,B.,Hopp,M.,Altankov,G., Groth,T.,“Membranes for biohybrid liver support system-investigations on hepatocyte attachment,morphology and growth”,Biomaterials,23,2467-2478,2002

4. Haydon,D.A.,Seaman,G.V.“Electrokinetic studies on the ultrastructure of human erythrocyte Ι.Electrophoresis at high ionic strengh-the cell as a polyanion”, Arch Biochem Biophys.,122, 126-136,1967

7. Dupin E,Le Douarin NM.“Development of melanocyte precursors from the vertebrate neural crest”.Oncogene ,22, 3016-3023,2003

國際替代計量

不同細胞培養在親水性及疏水性高分子薄膜上電泳行為之研究

全文下載

主題瀏覽