自製無隔膜電解水應用於抑制蝴蝶蘭主要病原菌

Application of Self-Produced Membraneless Electrolyzed Water on Suppression of the Major Pathogens of Phalaenopsis

王奕程(Yi-Cheng Wang)

指導教授 ： 方煒

國立臺灣大學/生物資源暨農學院/生物產業機電工程學研究所/碩士(2008年)

https://doi.org/10.6342/NTU.2008.02059

摘要

本研究針對包括電解質濃度、電解時間、電解電壓、溶劑種類與電極極距等不同電解條件下所製備之無隔膜電解水溶液的pH值、ORP 與FAC (自由氯濃度)進行分析。結果顯示使用氯化鉀為電解質、使用去離子水為溶劑、高電解質濃度與低電壓的條件下，單位電能轉換成FAC的效率為最高。考慮無隔膜電解水的高導電度可能造成在植物澆灌上的使用限制，本研究亦建立無鹽電解水的製備方法。
將自製之無隔膜電解水應用在蝴蝶蘭五種主要病原菌的滅菌上，測試的菌種包括細菌性軟腐菌、灰黴病、白絹病、疫病與黃葉病。各病原菌滅菌所需最低 FAC 濃度分別為60、30、60~120、120~200 與120 ppm。五種病原菌當中，以灰黴病病原菌的耐受性最低，只需要自由氯濃度為 30 ppm之無隔膜電解水就可抑制孢子發芽率達99 %以上。
本研究使用電化學循環伏安法定量分析FAC濃度，研究發現位於1,100 mV位置之氧化峰電流值與次氯酸鈉溶液濃度（10 ~ 1000 mM）有極高的線性關係。此線性關係亦存在於以氯化鉀做電解質之無隔膜電解水，其迴歸公式簡列如下：
Y = 18.509 * X
其中， X 為以掃描速率50 mV/s進行循環伏安法分析，其位於1100 mV之氧化峰值電流值，單位為μA。Y則為FAC之濃度，單位為ppm。
本研究已成功的建立了利用循環伏安法分析以氯化鉀為電解質所製備之無隔膜電解水之FAC濃度。對應於五種病原菌滅菌所需之最低FAC濃度值，若循環伏安法位於1,100 mV 之氧化峰電流值分別高於3.24、1.62、3.24~6.48、6.48~10.81與6.48 μA，則該無隔膜電解水對五種病原菌皆可達到99 %以上的滅菌效果。

關鍵字

無隔膜電解水 ； 循環伏安法 ； 蝴蝶蘭病原菌

並列摘要

Impacts of various electrolysis conditions on pH, ORP and FAC (free active chlorine) were investigated for the production of membraneless electrolyzed water, including electrolyte concentration, voltage, time elapsed, types of solvent and distance of electrodes. Results showed that condition of using KCl as the solute, using de-ionized water as solvent, high electrolyte concentration and low electrolyzed voltage has the highest efficiency in producing FAC in terms of electricity cost. Membraneless electrolyzed water has high EC, thus making it not suitable for irrigation. Method to produce saltless electrolyzed water with low EC was introduced.
Self-produced membraneless electrolyzed water was applied to the suppression of 5 major pathogens of Phalaenopsis. The lowest FAC concentrations required to kill pathogens of soft rot, botrytis blight, southern blight, phytophthora rot, and yellow rot were identified. They are: 60, 30, 60~120, 120~200 and 120 ppm, respectively. Among those pathogens, pathogen of botrytis blight required the lowest FAC concentration (30 ppm) to reach 99 % disinfection effect.
Cyclic voltammetry was used to analyze the FAC concentration of the electrolyzed water. The linear relationship of oxidation peak current at 1100 mV and FAC concentration of sodium hypochlorine (NaClO) from 10 to 1000 mM were established. Same linear relationship can be found with the membraneless electrolyzed water using KCl as the solute. The equation is listed as follows:
Y = 18.509 * X
where X is the oxidation peak current in μA at 1100 mV at 50 mV/s sweep rate and Y is the FAC concentration in ppm.
Based on this finding, measurement of FAC value can be greatly simplified. The lowest oxidation peak current at 1100 mV of membraneless electrolyzed water to kill 99% of the above listed pathogens are 3.24, 1.62, 3.24~6.48, 6.48~10.81 and 6.48 μA, respectively.

並列關鍵字

membraneless electrolyzed water ； cyclic voltammetry ； pathogens of Phalaenopsis

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