阿拉伯芥AtbHLH147基因功能性分析

Functional analysis of AtbHLH147 in Arabidopsis

王科晶(Ko-Ching Wang)

指導教授 ： 吳克強

國立臺灣大學/生命科學院/植物科學研究所/碩士(2012年)

https://doi.org/10.6342/NTU.2012.10866

摘要

無資料

關鍵字

阿拉伯芥 ； bHLH蛋白 ； 生長素 ； 吉貝素 ； 葉綠素 ； 光型態發生

並列摘要

Transcriptomic adjustment plays an important role in Arabidopsis de-etiolation in response to environmental signals. The G-box cis-element is commonly present in the promoters of these genes that respond positively or negatively to the light signal. By using yeast two hybrid screening, a basic helix-loop-helix transcriptional regulator bHLH147 was identified to interact with a histone deacetylase HDA15. Analysis of the bhlh147 mutant revealed that bHLH147 may function in cell proliferation especially in stem elongation and root development, and act as a positive regulator in chlorophyll biosynthesis. Chromatin immunoprecipitation assays revealed that bHLH147 bind to the promoters of the chlorophyll biosynthesis genes GUN5, PSAE-1, PSBQ and LHCB2.2 to regulate plant photomorthogenesis. This study provided evidence indicating that bHLH147 plays versatile roles in control plant development, including stem elongation, root development, hypocotyl elongation, and chlorophyll biosynthesis in Arabidopsis.

並列關鍵字

Arabidopsis ； bHLH147 ； Auxin ； Gibberellin ； Chlorophyll ； Photomorphogenesis

參考文獻

Alabadi, D., Gallego-Bartolome, J., Orlando, L., Garcia-Carcel, L., Rubio, V., Martinez, C., Frigerio, M., Iglesias-Pedraz, J.M., Espinosa, A., Deng, X.W., and Blazquez, M.A. (2007). Gibberellins modulate light signaling pathways to prevent Arabidopsis seedling de-etiolation in darkness. The Plant Journal 53, 324-335.

Alabadi, D. (2004). Gibberellins Repress Photomorphogenesis in Darkness. Plant Physiology 134, 1050-1057.

Alabad′, D, and ′zquez, M.A.B. (2009). Molecular interactions between light and hormone signaling to control plant growth. Plant Mol Biol 69, 409-17

Achard, P. (2003). Ethylene Regulates Arabidopsis Development via the Modulation of DELLA Protein Growth Repressor Function. The Plant Cell 15, 2816-2825.

Bernhardt, C. (2003). The bHLH genes GLABRA3 (GL3) andENHANCER OF GLABRA3 (EGL3) specify epidermal cell fate in the Arabidopsis root. Development 130, 6431-6439.