功能性金奈米粒子之合成與應用

金奈米粒子(gold nanoparticles, Au NPs)具有獨特光學特性、高穩定性、表面積大與良好生物相容性，故其作為催化劑、生物細胞顯影劑及化學和生化感測器方面，擁有相當大的潛力。本論文主要利用不同方法合成之金奈米粒子，探討其不同尺寸及表面修飾後之化學與光學特性的變化，並應用於生物感測器。論文共分為五個章節:第一章回顧金奈米粒子與螢光金奈米量子點(gold nanodots, Au NDs)的發展、合成、光學特性及應用。第二章藉由調控表面修飾之硫醇分子種類、密度及總量來改變合成11-巰基十一酸金奈米量子點(11-MUA–Au NDs)的速度及調控其螢光強度。第三章，內容是將(11-巰基十一烷基)-N, N, N-三甲基溴化銨 [(11-Mercaptoundecyl)-N,N-trimethylammonium bromide, 11-MUTAB] 修飾的金奈米量子點 (11-MUTAB–Au NDs)經過超音波震盪法處理，簡單調控金奈米量子點的大小、表面氧化態、硫醇分子修飾密度及螢光強度。第四章探討在紫外光照射的協助下調控表面不同硫醇分子的密度與比例，來改變金奈米量子點的合成速率及放光波長和強度。第五章，則是利用兒茶素合成金奈米粒子，修飾上適合體(aptamer)金奈米粒子，此奈米複合物可辨認腫瘤組織上目標蛋白質，並利用質譜儀所得到之金原子簇訊號來製成質譜顯影圖，可準確、快速的用來標定癌細胞及癌組織。

關鍵字

金奈米粒子；螢光金奈米量子點；光學特性；生物感測器

並列摘要

Gold nanoparticles (Au NPs) have been widely employed as catalysts, biosensors and as bioimaging agents due to their unique optical properties, high stability, large surface area, and high biocompatibility. This thesis focuses on the preparation, characterization, and application of Au NPs with different sizes. It is divided into five chapters. We review the background of Au NPs and photoluminescent gold nanodots (Au NDs) including their synthetic routes, optical properties and applications in detail. Our findings reveal that the photoluminescence of Au NDs depends on surface ligand density, ligand chain length and electron donation capability of alkanethiols, and also on the core size of the particles in Chapter 2. We developed a simple route to control the size, oxidation state, surface ligand density and photoluminescence (PL) of these (11-mercaptoundecyl)-N,N,N-trimethylammonium bromide–capped gold nanodots (11-MUTAB–Au NDs) through ultrasonic treatment in Chapter 3. In Chapter 4, our results indicate that the PL and reaction rate of 11-MUA assembly on the 11-MUTAB–Au NDs (11-MUA/11-MUTABAu NDs) could be controlled by accelerating the assembly of surface thiol ligands via UV irradiation. We report a simple, rapid and high-throughput cancer diagnosis system using functional nanoparticles consisting of poly(catechin) capped-gold nanoparticles (Au@PC NPs) and nucleoli-binding aptamer (AS1411)-conjugated small gold NPs (AS1411–Au NPs) in Chapter 5. The AS1411–Au NPs/Au@PC NPs can be used as a targeting agent in laser desorption/ionization mass spectrometry (LDI-MS)-based tumor tissue/cancer cell imaging via aptamer-protein recognition.

並列關鍵字

Gold Nanoparticles ； Gold Nanodots ； Optical Properties ； Biosensor

參考文獻

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功能性金奈米粒子之合成與應用

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