內向性整流型鉀離子通道Kir6.2之表現與純化

Expression and purification of the Kir6.2 channels

許碧絨(Pi-Jung Hsu)

指導教授 ： 樓國隆

國立臺灣大學/醫學院/口腔生物科學研究所/碩士(2005年)

https://doi.org/10.6342/NTU.2005.02055

摘要

內向性整流型鉀離子通道Kir6.2 (Inwardly rectifying potassium channels; Kir6.2)屬於Kir離子通道中的第六族，廣泛分布於身體不同的組織，特別在腦部、骨骼肌和胰臟β細胞中最多，並且調控許多重要生理功能如維持細胞靜止膜電位、胰島素分泌以及骨骼肌的收縮等。近十年的研究已知SUR (sulphonylurea
receptor)分子與調控Kir6.2的開啟有關，根據電生理的研究分析而知 ATP、MgADP、PIP2 (phosphatidyl-inositol-4,5 bisphosphate)也與調節Kir6.2有關，但無法得知分子間細部的作用機制，因此計劃進行蛋白質晶體結構測定。由於晶體結構測定需要較長的研究進行，所以本論文的目的在於表現與純化出Kir6.2離子通道的蛋白質，以作為進行蛋白質晶體結構測定之前期作業。
目前我們建構了Kir6.2離子通道與His-tag之融合蛋白，並表現於大腸桿菌系統中。根據電泳與西方點墨法結果顯示其蛋白已成功表現並得正確分子量。有趣的是施以高濃度的尿素無法將pellet的蛋白萃取出來，這種獨特的現象可能表示蛋白嚴重錯誤的疊合，因此進一步的純化以及使蛋白再正確疊合的處理為未來的首要目標。

關鍵字

融合蛋白 ； 內向性整流型鉀離子通道

並列摘要

Inwardly rectifying potassium channels Kir6.2 belong to the sixth family of Kir potassium channels. They are widely distributed in various tissues, especially in brain, skeletal muscles, and pancreatic β-cells. They regulate many important physiological functions, including the maintenance of resting potentials, the secretion of insulin and the contraction of skeletal muscles. The sulphonylurea receptor (SUR) subunit has been reported since a decade involving the regulation of the opening of Kir6.2 channels. In addition, such gating behavior was also suggested to be mediated by ATP, MgADP, and PIP2 based upon the electrophysiological analysis. The detailed mechanism remains to be identified. Therefore, we would like to, as a long-term goal, determine the crystal structure of the Kir6.2 channels with the expression and purification as pioneering studies presented in the thesis.

We have constructed the His-tag fusion protein of Kir6.2 channels and expressed with E. coli system. Results from electrophoresis and western blotting suggested successful expression of desired proteins in correct molecular weight. Interestingly, treatment of high concentration urea did not extract our protein from the pellets. Such unique phenomenon may imply a severe misfolding problem. Therefore, further purification and refolding treatment should the next leading concern in the near future.

並列關鍵字

Inwardly rectifying potassium channels ； fusion protein

參考文獻

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