九孔血液細胞凝集反應中細胞內訊息傳導路徑之研究

無脊椎動物血球細胞凝集反應為傷口修補及止血之重要機制。本研究以九孔 (Haliotis diversicolor) 的血液細胞為材料，研究血球在凝集反應中的細胞內訊息傳遞機制以及參與分子。實驗結果顯示，類親半乳醣蛋白galectin-like分子可能參與九孔血球細胞的凝集反應，且九孔血液細胞凝集反應需要細胞外鈣離子的存在。而在細胞內訊息傳遞的部分，使用PMA活化整體蛋白激脢C、或使用U73122抑制磷脂脢C或使用Gö6976抑制典型蛋白激脢C都會促進九孔血球細胞凝集反應；然而，使用細胞內鈣離子載體A23187或使用D-sphingosine抑制整體蛋白激脢C會抑制九孔血球細胞凝集反應，顯示和蛋白激脢C相關的訊息途徑參與凝集反應，並且至少有兩類以上，作用相異的蛋白激脢C參與其中。整體而言，細胞內整體磷酸化情況隨細胞凝集過程而增加。而由蝦得到的類TG蛋白質STG1亦對九孔血球細胞凝集反應有引發作用。由本實驗結果推論，galectin在細胞間辨識以及之後的細胞間連結反應上皆扮演重要角色，而至少兩類PKC在九孔血液細胞凝集反應的細胞內訊息傳遞路徑上則扮演著正負兩方雙面調控的角色。

關鍵字

九孔；無脊椎動物血球細胞；血球細胞凝集反應；蛋白激脢C ；磷脂脢C ；親半乳醣蛋白galectin

並列摘要

Hemocyte aggregation is the main function for wound repair and stop bleeding. In this study, hemocytes of abalone (Haliotis diversicolor) were used to investigate the mechanisms of intracellular signaling transduction on cell aggregation. Based on the results, galectin, one of galactose-sepecific and divalent cation-dependent binding proteins is involved to the aggregation of abalone hemocytes. About the signaling transduction, PMA(phorbol 12-myristate 13-acetate), a broad spectrum PKC(protein kinase C) activator、U73122, a PLC(phospholipase C) inhibitor, Gö6976, classical PKC inhibitor, significantly promoted the aggregation of abalone hemocytes; Contrarily, calcium ionophore A23187 and D-sphingosine, a broad spectrum PKC inhibitor inhibited the aggregation of abalone hemocytes. It is inferred that there are more than one type of PKCs involved in the aggregation of abalone hemocytes. During the period of aggregation of hemocytes, the total phosphorylation level of the hemocyte proteins is increased. In addition, one of STG(shrimp transglutaminase), STG1, secreted from shrimp(Marsupenaeus japonicus) hemocytes could influence the aggregation of abalone hemocytes.

並列關鍵字

abalone ； hemocyte ； cell aggregation ； PKC ； PLC ； galectin

參考文獻

Chen, J. H.,H. Y. Yang, S. W. Peng, Y. J. Chen, and K. Y. Tsai (1996) Characterization of abalone(Haliotis diversicolor) hemocytes in vitro. Biol. Bull. NTNU 31(1): 31-38.

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國際替代計量

九孔血液細胞凝集反應中細胞內訊息傳導路徑之研究

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