摘要
本研究目的為了解菌株Achromobacter xylosoxidans對於碳氮去除情形，並證明其具有同時硝化脫硝現象的存在，分別以懸浮菌株、單獨固定菌株、混合固定菌株及個別固定菌株進行批次試驗，以了解其同時硝化脫硝特性。此外，進行固定化菌株連續流試驗，評估其去除含氮廢水的可行性，並找出最佳同時硝化脫硝效率的操作條件，以提供實際廢水處理時之參考。 以自動監測連線之矽膠管供氧反應槽進行批次試驗，試驗結果可證實菌株Achromobacter xylosoxidans具有同時硝化脫硝功能，而經固定化後之菌株可有效縮短遲滯期，COD、氨氮去除率及硝化效率較懸浮菌株高。此外，為提升硝化效率，分別將自營硝化菌及異營硝化脫硝菌固定化，並且發現以混合固定方式的碳氮去除效果較佳，硝化效率亦有效提升。 於連續流操作下探討不同固定化方式、曝氣量、水力停留時間、碳氮比以及無機碳源對碳氮去除及硝化效率的影響，結果發現以混合固定菌株、大曝氣量(2.6 L/min)、較長之水力停留時間(10 hr)、適當的碳氮比(C/N=12.7)、添加碳酸氫鈉1 g/L可有較佳的硝化效率及氨氮去除率。
並列摘要
The objectives of this study were intended to investigate the effects of Achromobacter xylosoxidans on the COD and ammonia removals, and to verify the simultaneous nitrification and denitrification (SND). The SND reactions of suspended cells, immobilized cells, mixed-immobilized cells and individual-immobilized cells in batch reactors were studied. Furthermore, the availability of removing ammonia from the synthetic wastewater in continuous-flow system was evaluated to find the optimum operation of SND reactions, and to provide a guideline for the treating wastewater. Continuously monitoring batch reactor with silicone tubing was used to verify the SND ability of Achromobacter xylosoxidans. It was suggested that the lag phase of immobilized cells was shortened and the COD and ammonia removal efficiencies and nitrification were improved. Achromobacter xylosoxidans and autotrophic nitrifying sludge were immobilized individually and collectively to enhance nitrification efficiency, and it was observed that the mixed-immobilized cells demonstrated better nitrification efficiency and COD and ammonia removal efficiencies. The results of this investigation revealed that the mixed-immobilized cells, large aerating rate (2.6 L/min), long-term HRT (10 hr), C/N=12.7, and sodium bicarbonate added 1g/L were effective in removing ammonia and improving nitrification efficiency.