金屬離子與合成聚乙醚精土複合物毒性分析研究

The Toxicity Study of Metal Ion and Synthesized Polyether-Clay Compound

萬育仁(yu-ren wan)

指導教授 ： 李源弘

共同指導教授 ： 陳博彥(Bor-Yann Chen)

國立臺灣大學/工學院/材料科學與工程學研究所/碩士(2005年)

https://doi.org/10.6342/NTU.2005.02299

摘要

先前馮[2003]之研究(1)中發現，以麵包酵母菌(Saccharomyces cerevisiae CCRC 22049)作為藥物載體之蒙脫石(Montmorillonite))生物毒性評估，其與「蒙脫石對大鼠生物毒性實驗」結果均證明蒙脫石(Montmorillonite)基本上可視其為低毒性具可行性之生物材料，故本研究利用麵包酵母之生物毒性評估法檢測常見之一般生醫「植入」材料(如:F136 【Ti-6Al-4V】 、ASTM F75【Co-Cr-Mo】、F138 316L不鏽鋼【Fe-Ni-Cr】)以及合成之聚乙醚精土複合物(synthesized polyether-clay compound)為對象，並與文獻上之毒性結果作成份分析比較，以進一步判斷麵包酵母菌替代文獻中生物毒性評估法之可行性，再就新複合材料之毒理性作探討。研究首先，以合金中八種主要元素作單成分生物毒性分析，後續再就成份間交互作用(如: 協同作用【synergism】拮抗作用【antagonism】)以分別定義出主要毒性金屬及其對於合金成分之效應，並以毒理學上之「劑量反應分析」(dose-response analysis)方式作為研究評估分析之學理依據，試圖對生醫材料之安全與功能改善進行深入之探討。而後就合成之複合物作生物毒性分析結果探討。

關鍵字

生醫材料 ； 麵包酵母菌 ； 劑量反應分析 ； 聚乙醚精土複合物

並列摘要

Our previous study(1) indicated that the results of toxicity assay for montmorillonite as drug carrier in bacterial(Saccharomyces cerevisiae CCRC 22049) and rat model pointed out that the toxicity of the drug was very weak. Therefore, this study focused the toxicity of three popular biomaterials (example:ASTM F136 【Ti-6Al-4V】、ASTM F75【Co-Cr-Mo】、ASTM F138 316L stainless steel【Fe-Ni-Cr】) and synthesized polyether-clay compound in bacterial (Saccharomyces cerevisiae) assay. The toxicity of eight aqueous metal solutions was first deteremined. Then, aqueous metal solutions were then mixed according to the proportions of the elements found in these alloys, and their toxicity was measured with yeast. Mixture behavior was classified as synergistic or antagonistic by comparing measured toxicity to predicted toxicity. Then measured toxicity of biomaterial and synthesized polyether-clay compound was analyzed by dose-response analysis and compared with measured toxicity by Microtox(from reference(13)). In the result, the experiments demonstrate the safty and improvement of materials and discuss these.

並列關鍵字

biomaterial ； Saccharomyces cerevisiae ； dose-responseanalysis ； synthesized polyether-clay compound

參考文獻

(1)馮義凱”蒙脫石對酵母菌反應機制及其高劑量下對大鼠急性毒理學與懷孕之影響”國立台灣大學材料科學與工程學研究所,碩士論文,中華民國九十三年七月

(4)F.H. Lin, Y.H. Lee, C.H. Jian, Jau-Min Wong, Ming-Jium Shieh, Cheng-Yi Wang ” A study of purified montmorillonite intercalated with 5-fluorouracil as drug carrier” Biomaterials 23 (2002) 1981–1987

(5)Ying Mu, Takeshi Kobayashi, Masae Sumita, Akiko Yamamoto, Takao Hanawa” Metal ion release from titanium with active oxygen species generated by rat macrophages in vitro” J Biomed Mater Res. 2000 Feb 49(2):238-43.

(6) H. Tomas, G. S. Caevalho, M. H. Fernandes, A. P. Freire, and L. M. Abrantes, “Effects of Co-Cr corrosion products and corresponding separate metal ions on human osteoblast-like cell cultures” Journal of Materials Science: Materials in Medicine, 7, 291-296, 1996.

(7)P. R. Bouchard, B. Jonathan, B. A. Albrecht, R. E. Kaderly, J. O. Galante, and B. U. Pauli, “Carcinogenicity of CoCrMo(F-75) implants in the rat” J. Biomed. Mater. Res., 32, 37-44, 1996.