高靈敏度之壓阻式微懸臂梁生物感測器應用於蛋白質分子之即時檢測

Use of Piezoresistive Microcantilever Biosensor to Real-Time Monitor Bio-molecules

游育諺(Yuh-Yan Yu)

指導教授 ： 黃榮山

國立臺灣大學/工學院/應用力學研究所/碩士(2006年)

https://doi.org/10.6342/NTU.2006.02471

摘要

以目前生醫檢測系統來說，其高成本、檢測時間長、佔空間與無法符合即時監測為主要的缺點。而隨著生物奈米技術的快速發展，生物感測工具的設計與發展必須趨向於微小化、高靈敏度、可攜帶式以及可無線傳輸功能等。
而市面上使用的微生化感測器大多利用化學方法或螢光標定進行檢測，且須架設光場，來進行檢測，本研究將以力學為基礎發展壓阻式微懸臂梁感測器(Piezoresistive micro-cantilever biosensor)進行生物檢測，以取代化學與螢光標定之檢測方法。但因光源不易聚焦於微懸臂梁上，檢測出來的訊號易產生極大誤差；本文擬以壓阻式微懸臂梁檢測方式取代傳統光學檢測。藉由壓阻式微懸臂梁建構在惠斯登電橋電路(Wheatstone bridge circuit)上作為轉換機制，先將生物與化學的反應過程轉換為奈米力學的變形運動，此變形經過壓阻效應可轉換成電壓訊號。當生物分子在壓阻式微懸臂梁生物晶片的表面結合時，會引入表面應力使其彎曲，約在數十個奈米左右，藉由電阻的改變而傳出訊號，經放大電路放大訊號。再經無線傳輸系統單晶片將訊號傳出到電腦分析，以達到遠端即時診斷的目的。
在本研究中已成功的利用微機電製程製作出壓阻式微臂梁生物感測器，經由無線傳輸的方式量測出自組裝分子(bio-linker)與抗體(Anti-CRP)的訊號。而以壓阻式微懸臂梁生物感測器之架構具有微小化、低成本、高功能性、高效率與可攜式的優點。未來將可繼續朝向系統晶片(System-on-chip)設計與無線感測的目標繼續往前邁進。

關鍵字

壓阻式微懸臂梁感測器 ； 惠斯登電橋電路 ； 系統晶片

並列摘要

Bio-sensing tools have been moving towards miniaturization, high sensitivity, portability and wireless networking. While fluorescent labeling of nucleic acids is becoming a standard procedure, protein labeling techniques are not yet as well established. The purpose of this paper is to demonstrate a novel reusable biosensor which achieves the continuous label-free recognition of biological substances in real-time, which based on the nanomechanics of a piezoresistive microcantilever.
Over the past few years, an increasingly evident number of studies have been conducted on using the microcantilevers as transducers in biochemical-sensing systems. In principle, adsorption of biochemical species on a functionalized surface of a micro-cantilever will cause bio-induced surface stress and accordingly the cantilever bends. We used this technique in the immunoassay.
A microcantilever based biosensor with piezoresistive has been developed using surface micromachining technique in this paper, which is cost effective, miniaturized and high applicability. This provides a novel method to detect bio-molecules which is due to molecule binding induced cantilever deflection. We have demonstration of wireless real-time label-free detection of bio-linker and anti-CRP antibody, the signal was successfully detected by using piezoresistive cantilever biosensor. The tendency of cantilever deflection is agreement with optical-based cantilever detection. In the future, the piezoresistive cantilever biosensor will offer a powerful plateform for high-throughput bioassays in proteomics and immunology.

並列關鍵字

Piezoresistive ； surface micromachining ； Biosensor

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