台灣牛隻副結核病的傳播風險因子和控制策略

Transmission Risk Factor and Control Strategy of Bovine Paratuberculosis in Taiwan

李文達(Wen-Ta Li)

指導教授 ： 蕭世烜

共同指導教授 ： 鄭謙仁 ； 龐飛

國立臺灣大學/獸醫專業學院/獸醫學研究所/碩士(2010年)

https://doi.org/10.6342/NTU.2010.03064

摘要

無資料

關鍵字

控制計畫 ； 血清酵素結合免疫吸附法 ； 副結核病 ； 即時定量聚合 ； 監測 ； 異數重複序列

並列摘要

Bovine paratuberculosis is caused by Mycobacterium avium subsp. paratuberculosis (MAP). The aims of this study was to investigate the current MAP infection status and MAP transmission risk factors in Taiwan; thereby, to develop a practical control program. A nation-wise survey by serum enzyme linked immunosorbent assay (ELISA) was conducted. Environmental samples from dairy herds were collected for detecting MAP by real time PCR and for phylogenic analysis by variable number tandem repeats (VNTRs). Results indicated that MAP seroprevalence in dairy cow was 10.1%, and 66.7% dairy herds were positive, MAP seroprevalence of beef cattle was 2.8%, MAP seroprevalence of intradermal tuberculin test (ITT) positive cattle was 51.4%. MAP infection status and transmission risk factors are different among different herds. Soil and feces were high risk carries for transmitting MAP. Nine substrains of MAPs were identified indicating direct link of MAP transmission among herds. According to the results above and previous research, a MAP control program was developed.

並列關鍵字

control program ； ELISA ； paratuberculosis ； real-time PCR ； survey ； VNTRs

參考文獻

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