應用雙光子顯微術觀測除毛膏對皮膚之分子穿透增益之影響

Utilizing Two-photon Microscopy to Investigate the Effects of Depilatory Agent as a Transdermal Penetration Enhancer

李今寧(Jin-Ning Lee)

指導教授 ： 董成淵

國立臺灣大學/理學院/物理研究所/碩士(2007年)

https://doi.org/10.6342/NTU.2007.02606

摘要

摘要
本篇文章目的在於：利用雙光子顯微術，探討除毛膏如何改變皮膚表面的角質層結構以增益經皮滲透。將人類包皮塗上除毛膏十分鐘後清洗掉，利用水溶性與脂溶性的標的螢光劑模擬藥物分子經皮滲透的情況。然後，利用雙光子激發皮膚的自發螢光、H&E染色切片、Nile red 染色切片與穿透式電子顯微術觀察除毛膏對角質層產生的影響。

實驗結果顯示除毛膏對於水溶性與脂溶性的分子，都有增益經皮滲透的結果；利用雙光子顯微術所拮取的皮膚自發螢光影像，可以辨別出角質層有均質化或是較為空洞的情況。傳統的H&E染色切片可以看到角質層有些許脫落而變薄；Nile red 螢光染劑針對角質層之角質細胞間的脂質層，亦顯示細胞間的脂質多層薄片結構受到除毛膏影響而發生斷裂。超顯微影像技術--穿透式電子顯微術觀察皮膚角質層結構，可以佐證前面的實驗結果。

所以由以上實驗結果顯示，除毛膏除了會破壞角質細胞內的角質素外，連帶的細胞間的脂質也隨之毀壞。因此，可以推論藥物可能同時由細胞間隙與細胞內滲透進入皮膚內。

關鍵字

雙光子 ； 皮膚 ； 除毛膏

並列摘要

Abstract
The aim of this research is to characterize the penetration enhancing effect of depilatory agent, and to investigate the change of stratum corneum(SC). Human foreskin was treated by a depilatory for 10 minutes and then using fluorescent probe, hydrophilic sulforhodamine (SRB) and hydrophobic rhodamine B hexyl ester (RBHE), to model drugs penetration that was quantified by twophoton microscopy. The structural alternations of SC are assessed by routine histology, transmission electron microscopy and Nile red staining.

The results showed that the penetration of both hydrophilic and hydrophobic fluorescent probes could be enhanced. Nile red staining revealed, instead of a regular lipid layers around the brick of corneocytes, a disorganized and homogenized pattern of lipid distribution. Ultrastructural analysis showed that the protein envelope of coenocytes disintegrated, especially intercellular lipid of SC. We concluded that depilatory agent enhance drug penetration by disrupting both the cellular integrity of corneocytes and the regular packing of intercellular lipid of SC.

並列關鍵字

multiphoton ； transdermal penetration ； skin ； depilatory

參考文獻

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