在巨噬細胞和小神經膠質細胞中，oregonin對於iNOS表現的抑制作用與其抑制NF-kappa B及引發HO-1之相關性探討

Inhibitory effects of oregonin on inducible nitric oxide synthase expression in macrophages and microglial cells: involvement of NF-kappa B inhibition and heme oxygenase-1 induction

李晟睿(Cheng-Jui Lee)

指導教授 ： 林琬琬

國立臺灣大學/醫學院/藥理學研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.10320

摘要

從台灣赤楊分離而得的oregonin 是一種diarylheptanoid 的衍生物，已知具有抗氧化與抗發炎的作用。雖然之前的報告已經說明過oregonin可以抑制iNOS 和 COX-2的蛋白表現，但是截至目前為止，其詳細的作用機制依然不清楚。在本研究中，我們更進一步的去解釋oregonin對於iNOS、COX-2的抑制作用，以及HO-1的誘發作用，以釐清其抗發炎作用的分子機制。我們的結果顯示，在老鼠的巨噬細胞株RAW264.7與小神經膠質細胞株BV-2中，oregonin可以減少LPS或IFN-

關鍵字

誘發性一氧化氮合成酶

並列摘要

Oregonin isolated from Alnus formosana is a diarylheptanoid derivative, which appears to have antioxidative and anti-inflammatory activities. Previous reports have demonstrated that oregonin is able to inhibit iNOS and COX-2 expression. Up to date the precise mechanism responsible for inhibition of iNOS and COX-2 by oregonin remains unclear. In this study, we further elucidated actions of oregonin on the iNOS, COX-2, and HO-1 induction in order to clarify the molecular mechanism underlying its anti-inflammatory actions. Our results showed that oregonin reduced NO production induced by LPS or IFN-

並列關鍵字

iNOS

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