紅血球生成素及其受器於口腔鱗狀細胞癌之表現

Expression of erythropoietin and its receptor in oral squamous cell carcinomas

邱正軒(Cheng-Hsuan Chiu)

指導教授 ： 江俊斌

國立臺灣大學/醫學院/臨床牙醫學研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.02113

摘要

中文摘要

背景：紅血球生成素（Epo）是一個多功能生長因子，能促進細胞分裂、移動、存活、腫瘤血管新生以及腫瘤細胞的侵襲和轉移。Epo多樣性的生物功能，是經由和紅血球生成素受器(EpoR)結合所調控。

方法：本研究利用免疫組織化學染色法，探討Epo和EpoR於60例口腔鱗狀細胞癌（OSCC），40例口腔上皮變異（OED），20例口腔上皮過度角化 (OEH)，和10例正常口腔黏膜（NOM）之表現。並評估Epo和EpoR於OSCC、OED、OEH和NOM四組中表現之差異。並進一步利用統計分析，探討Epo和EpoR於OSCCS之表現和OSCC患者臨床病理參數及存活率之相關性，以研究是否Epo和EpoR 之表現和OSCC之腫瘤進展及預後有關。

結果：Epo和EpoR之陽性染色主要表現於上皮及腫瘤細胞之細胞質上。Epo平均標記指數，從NOM 之0%，至OEH 之14±10%，至OED之 45±24%，至OSCC 之68±21%，有統計學上有意義隨著惡性程度之逐步增加而增高之情形 (P=0.000)。就EpoR之平均標記指數而言，從NOM 之8±3%，至OEH 之30±13%，至OED之 53±27%，至OSCC 之74±23%，也有統計學上有意義隨著惡性程度之逐步增加而增高之情形(P=0.000)。統計分析結果顯示，Epo或EpoR在OSCC中之較高表現與較年輕的患者年齡、較大的OSCC腫瘤、有淋巴結轉移和較高之臨床期數，有統計學上有意義之相關。
結論：我們的結果顯示，Epo和EpoR之表現為台灣口腔癌形成之早期現象。Epo或EpoR在OSCC之標記指數可預測台灣OSCC的腫瘤進展。

關鍵字：紅血球生成素，紅血球生成素受器，口腔癌，口腔癌前病變。

關鍵字

紅血球生成素 ； 口腔癌 ； 口腔癌前病變 ； 紅血球生成素受器

並列摘要

Abstract
Background: Erythropoietin (Epo) is a multi-functional growth factor that promotes cell proliferation, migration and survival, tumor angiogenesis, and tumor cell invasion and metastasis. Its diverse biological effects are mediated through its interaction with its erythropoietin receptor (EpoR).
Methods: In this study, we examined the expression of Epo and EpoR in 60 specimens of OSCC, 40 specimens of oral epithelial dysplasia (OED), 20 specimens of oral epithelial hyperkeratosis (OEH), and 10 specimens of normal oral mucosa (NOM) by immunohistochemistry. The Epo and EpoR labeling indices (LIs) in OSCC, OED, OEH, and NOM groups were calculated and compared between groups. The correlation between the expression of Epo or EpoR in OSCCs and clinicopathological parameters or survival of OSCC patients was analyzed statistically to investigate the possible influence of Epo or EpoR on the progression and prognosis of OSCCs in Taiwan.
Results: Positive Epo or EpoR staining was mainly cytoplasmic. The mean Epo LI increased significantly from NOM (0%) through OEH (14±10%) and OED (45±24%) to OSCC (68±21%, P=0.000). The mean EpoR LI also increased significantly from NOM (8±3%) and OEH (30±13%) through OED (53±27%) to OSCC (74±23%, P=0.000). Statistical analysis showed a significant association of higher Epo or EpoR expression in OSCCs with the younger age of patients, the larger tumor size, the presence of lymph node metastases, and the more advanced clinical stages of OSCC.
Conclusion: Our results suggest that the expression of Epo and EpoR is an early event in oral carcinogenesis in Taiwan. The Epo or EpoR LI in OSCC samples can predict the progression of OSCCs in Taiwan.
Key words: Erythropoietin, erythropoietin receptor, oral cancer, oral precancer

並列關鍵字

erythropoietin ； erythropoietin receptor ； oral cancer ； oral precancer

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