應用石英晶體微天平於水解磷脂質在白蛋白自組裝單層膜上吸附行為之研究

A Study on Adsorption Behavior of Lysophosphatidic Acid on Albumin Self Assemble Monolayer using Quartz Crystal Microbalance

李靚蕙(Chin Hui Li)

指導教授 ： 黃義侑

國立臺灣大學/工學院/醫學工程學研究所/碩士(2006年)

https://doi.org/10.6342/NTU.2006.10496

摘要

摘要
水解磷脂質是一種構造十分簡單的磷脂質。由於水解磷脂質是訊息傳遞系統中相當重要的調節因子，因此與許多生理功能密切相關。水解磷脂質在人體中存在於血清、腦髓液及其他體液；在體內循環時則多數與血清白蛋白相結合。由於在許多研究中發現，部分疾病患者的血清水解磷脂質含量較正常個體有顯著的增加，因此關於水解磷脂質與其主要結合之蛋白質 – 血清白蛋白間之互動關係之研究，對於未來針對水解磷脂質之親和材料的發展與設計自有其貢獻。然而在過去的研究中，多數的研究著重在水解磷脂質其生物性功能的研究，關於水解磷脂質與血清白蛋白之互動關係的研究較為少見，同時此類研究多只專注於兩者靜態親和關係與其他結合位競爭脂質的比較。在本研究中，利用可行即時動態監測的生物感測器 – 石英晶體微天平，於金電極上所固定之自組裝白蛋白單層膜偵測白蛋白對水解磷脂質的動態吸附行為。並配合phosphate capture complex加重水解磷脂質，以增加偵測之靈敏度。

關鍵字

水解磷脂質 ； 石英晶體微天平

並列摘要

Abstract
Lysophosphatidic acid (LPA) is a simple structure phospholipid. It plays a crucial role in signaling pathway which is related to numerous drown stream biological functions. LPA presents in serum, saliva, and other body fluids. In circulation, most LPA binds to serum albumin. Because of the notable increasing LPA concentration in clients with cancers, renal disease et al., the need to eliminate the excessive LPA in those populations is apparent. To understand the interaction relation between LPA and its carrier – serum albumin can contribute to the development of the bioaffinity materials to remove the LPA from circulation. However, the previous researches solely aim at the static affinity properties; the dynamic information remains unknown. In this study, we use a real time monitoring device – quartz crystal microbalance, to observe the dynamic adsorption behavior of LPA onto a self assembly albumin monolayer. Moreover, we combine the phosphate capture complex to weight the LPA and thus, increase the detection sensitivity of LPA.

並列關鍵字

Lysophosphatidic acid ； Quartz crystal microbalance

參考文獻

Gaits, F., O. Fourcade, et al. (1997). "Lysophosphatidic acid as a phospholipid mediator: pathways of synthesis." FEBS Lett 410(1): 54-8.

Goetzl, E. J. and S. An (1998). "Diversity of cellular receptors and functions for the lysophospholipid growth factors lysophosphatidic acid and sphingosine 1-phosphate." Faseb J 12(15): 1589-98.

Inoue, C. N., M. Epstein, et al. (1999). "Lysophosphatidic acid and mesangial cells: implications for renal diseases." Clin Sci (Lond) 96(4): 431-6.

Koh, J. S., W. Lieberthal, et al. (1998). "Lysophosphatidic acid is a major serum noncytokine survival factor for murine macrophages which acts via the phosphatidylinositol 3-kinase signaling pathway." J Clin Invest 102(4): 716-27.

Kue, P. F., J. S. Taub, et al. (2002). "Lysophosphatidic acid-regulated mitogenic ERK signaling in androgen-insensitive prostate cancer PC-3 cells." Int J Cancer 102(6): 572-9.