多參數石英晶體微天平與表面電漿共振整合生醫系統平台之研究與發展

Research and Development of An Integrated Multi-Parameter Quartz Crystal Microbalance and Surface Plasmon Resonance System

顏維廷(Wei-Ting Yen)

指導教授 ： 李世光 ； 吳文中

國立臺灣大學/工學院/工程科學及海洋工程學研究所/碩士(2007年)

https://doi.org/10.6342/NTU.2007.02921

摘要

生物分子間常有鍵結反應甚至構形的變化，由於其反應過程複雜，因此增添越多量測參數將有助於我們分析探討。而石英晶體微天平本身便已有頻率變化與消散係數兩個參數，因此本研究以石英晶體微天平為平台，結合表面電漿共振的光強變化，利用三個參數來觀察生物連結物與金膜鍵結的反應過程，並同時比較兩者的差異。
石英晶體微天平與表面電漿共振皆為發展相當成熟的生物感測器，雖然其感測原理不同，但卻皆有相當高的靈敏度，因此各自有其擅長的領域，同時也常被拿來比較。較常見的比較方式是分別量測，但容易受到環境變因而影響其準確性；另外也有同步量測，但其表面電漿共振是採用光柵耦合的方式，因此需要在石英金膜表面做蝕刻製程，較耗費時間與製作成本。
因此本研究是採直接且易於操作的方式，透過藕合油直接將石英晶體微天平與稜鏡疊合，利用稜鏡耦合來激發表面電漿共振，其中並將石英與稜鏡接觸的那面改成ITO電極。在不改變原始架構太多的情況下，使兩種生物感測器都能保持其原有的特性。

關鍵字

石英晶體微天平 ； 表面電漿共振

並列摘要

As binding and even conformation changes often appear in bio-molecule interactions, adding more measurement parameters can facilitate the analysis of such a complex reaction process. Quartz Crystal Microbalance(QCM) provides us with measurement capabilities on two such important parameters, i.e., frequency change and dissipation factor. In this study, Surface Plasma Resonance(SPR) was added to QCM to create a measuring system with the third parameter of interest. That is, optical power change from SPR can be simultaneously measured with frequency change and damping factor. In addition to observe the reaction process of bio-linker and gold film through this new construction. The difference between the QCM and SPR were compared as well.
QCM and SPR are both well-developed biosensors. They both have high sensitivity even though they were developed with different measurement principle. With each of the two methods possess their own advantageous research fields, their pros and cons were compared from time to time. The most common approach is to compare each of these two methods independently. However, this kind of comparison suffers from the influence of different environmental conditions. A better approach is to perform the comparisons in-situ, simultaneously, and side by side. It is to be noted that since SPR is typically activated by method of grating coupling, which demands lithorgraphic process on to the gold film deposited on the quartz. In other words, it will make the comparison time consuming and costly.
In this thesis, we developed a direct and easy to operate method to perform the above-mentioned comparisons. More specifically, we have integrated both QCM with SPR into a single device in this study. In our design, QCM is placed directly on the prism through coupling oil and SPR is activated by prism coupling. The electrode of quartz which contacts the prism is replaced with ITO for light beam transmissions. The simplicity provides us with a new instrument that maintains the benefit of both instruments, which simultanesously enables the measurement of integrated parameters for sensing and a platform for functional comparisions.

並列關鍵字

QCM ； SPR

參考文獻

秦慶瑤，「2007年生技產業回顧與展望」，生物技術開發中心，2008。

陳俊光，「同時解析分子質量與液相阻尼之相位補償式石英微質量天平」，國立台灣大學應用力學研究所博士論文，2007

陳逸豪，「手持式表面電漿共振儀的設計與研製」，國立台灣大學工程科學及海洋工程研究所碩士論文，2006

詹家銘，「圓偏光表面電漿子干涉儀之研製」，國立台灣大學應用力學研究 所碩士論文，2006

Rich RL, Myszka DG. Survey of the year 2005 commercial optical biosensor literature. J. Mol. Recognit. 19(6): 478–534, 2006.