自小花蔓澤蘭以膜分離及酵素水解生產木寡糖

Production of xylooligosaccharides from Mikania micrantha by membrane separation and enzymatic hydrolysis

詹璧庭(Pi-Ting Chan)

指導教授 ： 柯淳涵

國立臺灣大學/生物資源暨農學院/森林環境暨資源學研究所/碩士(2020年)

https://doi.org/10.6342/NTU202001954

摘要

小花蔓澤蘭(Mikania micrantha)為外來入侵蔓藤植物，已對海拔一千公尺以下之植物造成嚴重的影響，有「綠癌」之稱，目前農林單位已全力加以剷除中。然而其極高的生產力及廣泛的分布性，極易成為生質能源的良好原料。
木寡糖是一種機能性食品，能在較寬的pH值、溫度範圍內保持穩定，與其他寡糖相比較，木寡糖最難被人體消化;木寡糖能選擇性地增加直腸道中的益菌，改善腸道菌相。在日本，木寡糖被認為是最有前途的功能性寡糖之一，並已得到廣泛的應用。
本研究利用12% NaOH持溫60 oC與150rpm反應16小時進行小花蔓澤蘭粗聚戊醣萃取後，再以酒精沉澱法、奈米過濾法和酒精超膜過濾法製備三種小花蔓澤蘭木聚糖(EMX、NMX與EUMX)。結果顯示酒精沉澱法所得到的木聚糖純度較高但聚戊醣總量較少;利用膜過濾法可得到純度較低但較多的聚戊醣總量。
三種不同的小花蔓澤蘭木聚糖與商用樺木木聚糖(聚戊醣含量為0.9%)，以實驗室所分離的Paenibacillus campinensis BL 11木聚糖酶，在10、50、100 U/mL三種酵素劑量，持溫60 oC與150 rpm反應0-96小時的條件下進行水解，並以HPLC檢測木寡糖含量。
結果顯示奈米過濾法製備的小花蔓澤蘭木聚糖在50 U/mL木聚糖酶的條件下水解24小時後，與商用樺木木聚糖相較之下，可產出較多的木寡糖，其木寡糖產率分別為68%與59%。奈米過濾法之小花蔓澤蘭木聚糖水解後，主要產出的木寡糖為木六糖(X6)、木五糖(X5)及木二糖(X2)，含量分別為43%、21%及21%。

關鍵字:小花蔓澤蘭、木寡糖、Paenibacillus、膜過濾、奈米過濾

關鍵字

小花蔓澤蘭 ； 木寡糖 ； 纖維素酶 ； 膜過濾 ； 奈米過濾

並列摘要

Mikania micrantha (M. micrantha), “green cancer”, is one of the invasive vine plants; it already causes the serious effect of plants which are after an elevation of 1000 meters. Nowadays, the agricultural and forest organizations are making efforts to eradicate the alien plant. Because of its vigorous, fast and rampant growth habit, M. micrantha has the potential to be a good biomass raw material.
Xylooligosaccharides (XOs), one of the functional foods, it has a wide range of pH and temperatures stability. XOs is more non digestible than other oligosaccharides; it can proliferate beneficial bacteria and improve microflora in human gut. In Japan, XOs is considered as one of the most potential functional oligosaccharides, and already has rather extensive application.
In this study, extracted crude M. micrantha xylan(MX) with 12 %(w/v) NaOH solution at 150 rpm, 60 oC for 16 h., and then collected different kind of MX by ethanol-precipitation(EMX), nanofiltration(NMX) and ethanol-precipitation and ultrafiltration(EUMX). The result showed that ethanol -precipitation method could get purer xylan but less total pentosan weight, membrane filtration method was opposite to ethanol -precipitation method.
In XOs production, three kind of MX and commercial birchwood xylan(BX) (pentosan content: 0.9%) were hydrolyzed by adding 10, 50 and 100 U/mL Paenibacillus campinensis BL 11 xylanaes at 60 oC and shake (150 rpm) for 0-96 h; analyzed XOs by the HPLC.
The result showed that NMX which was hydrolyzed by 50 U/mL for 24h, could get more XOs than commercial BX, the yield of XOs were 68 and 59%, respectively. The major XOs products of NMX were xylohexaose(X6), xylopentaose(X5) and xylobiose(X2); the yields were 43, 21 and 21%.

Key word: Mikania micrantha, Xylooligosaccharides, Paenibacillus, Membrane filtration, Nanofiltration

並列關鍵字

Mikania micrantha ； Xylooligosaccharides ； Paenibacillus ； Paenibacillus, Membrane filtration ； Nanofiltration

參考文獻

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