生物可分解性多功能微粒體於肝癌栓塞治療之研究

Biodegradable and multifunctional microspheres for treatment of hepatoma through transarterial embolization

江秉芳(Ping-Fang Chiang)

指導教授 ： 謝銘鈞

國立臺灣大學/工學院/醫學工程學研究所/碩士(2016年)

https://doi.org/10.6342/NTU201610451

摘要

無資料

關鍵字

肝癌 ； 肝動脈栓塞 ； 微粒體 ； 艾黴素 ； 錸-188

並列摘要

The purpose of this study is to develop biodegradable microspheres which combined with chemotherapy and radiotherapy for transcatheter arterial embolization/chemoembolization (TAE/TACE) or local injectable forms. By locally and slowly release of chemotherapeutic agents, the effect of cancer treatment can be extended.
In the experiment, we used biodegradable and biocompatible polymer Poly(D,L-lactide-co-glycolide)(PLGA) to prepare micron particles. Through double emulsion, the microspheres contain water-soluble polymers, including carboxymethylcellulose sodium salt, poly(sodium 4-styrenesulfonate), poly(4-styrenesulfonic acid) ammonium salt solution and Poly(vinylsulfonic acid, sodium salt) solution. The functional groups of those polymers will dissociate into -SO3- or -COO- in water. Doxorubicin can be loaded into the beads using an interaction process between the protonated primary amine on the drug and the negatively charged groups. The microspheres could embedded with 188Re-tin-colloid in the pore spaces which on or in the particles.
Experimental results show that 50 mg PVSA/PLGA microspheres absorbed 2.5 mg doxorubicin within an hour had the best absorption efficiency. Microspheres were embedded with 188Re-tin-colloid and performed in a rat hepatocellular carcinoma model. NanoSPECT/CT imaging and bio-distribution showed the microspheres were still in the liver after 72 hours. The tumor growth was more profoundly inhibited by treatment with Re/DOX@MS than others by ultrasonography during 4 weeks observation period.
The microspheres may offer the advantage of locally injection or embolization. In the future, the drug delivery system for cancer radiotherapy combined with chemotherapy using a biodegradable PLGA microspheres consisting of therapeutic radionuclide and chemotherapeutic drugs could maximize their effects on hepatocellular carcinoma.

並列關鍵字

hepatocellular carcinoma ； transcatheter arterial embolization (TAE) ； microsphere ； doxorubicin ； 188Re

參考文獻

22. Argyrou M, Valassi A, Andreou M, Lyra M. Rhenium-188 Production in Hospitals, by W-188/Re-188 Generator, for Easy Use in Radionuclide Therapy. International Journal of Molecular Imaging, 2013. DOI:10.1155/2013/290750.

1. Pisani P, Parkin DM, Ferlay J. Estimates of the world wide mortality from eighteen major cancers in 1985. Implications for prevention and projections of future burden. Int J Cancer, 1993. 55(6): 891-903.

2. Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int. J. Cancer, 2010. 127(12): 2893-2917.

3. El-Serag HB. Hepatocellular carcinoma. New England J. Med, 2011. 365(12): 1118-1127.

4. Liu, L. X., Zhang WH, Jiang HC. Current treatment for liver metastases from colorectal cancer. World J. Gastroenterol, 2003. 9(2): 193-200.