中華鱉 (Pelodiscus sinensis)腦垂體糖蛋白激素
cDNA序列mRNA生成調控之分析

腦垂體糖蛋白激素促甲狀腺激素、促濾胞激素及促黃體激素，係由alpha及beta兩個不同的次單元所構成。在同一動物種別中，alpha次單元是相同的；而beta次單元則具有種別性與激素的特異性。目前這些激素cDNA的選殖及其調控研究，主要在哺乳類、鳥類、兩棲類及魚類，而爬行類則是闕如。為了能更瞭解脊椎動物腦垂體糖蛋白激素cDNA的核苷酸與胺基酸，在序列親緣上的多樣性，與mRNA生成的調控，本論文探討中華鱉(Pelodiscus sinensis)

關鍵字

甲釋素；性釋素；基因調控；基因選殖；次單元；甲狀腺素；演化；歧異度；次單元；腦垂體；糖蛋白激素；促甲狀腺激素；促濾胞激素；促黃體激素

並列摘要

The pituitary glycoprotein hormones (thyrotropin, TSH; follitropin, FSH; lutropin, LH) are composed of two dissimilar subunit molecules, alpha and beta subunits are identical in a species; while beta subunits are specific and determine the hormone activity and species specificity. The comparative studies of cloning of cDNAs encoding these hormones and their gene regulations have been extensively conducted in mammals, birds, amphibian and fish. However, virtually no information has been available in reptiles. Reptiles occupy a key position in evolutionary history of vertebrates as the birds and mammals evolved from them. For better understanding the phylogenetic diversity and evolution of the cDNA nucleotide and peptide sequences of pituitary glycoprotein hormones in vertebrates, a study was conducted to clone the cDNAs of alpha subunit and the beta subunits of TSH, LH, and FSH of the Chinese soft-shell turtle, Pelodiscus sinensis, and to investigate the regulation of gene expression. The cDNAs were cloned by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods. The cloned alpha cDNA consists of 670-bp nucleotides, including 71-bp 5’-untranslated region (UTR), 363-bp open reading frame, and 3’-UTR of 219-bp nucleotides, following by a 17-bp poly A trait. It encodes a 120 amino acid precursor molecule of alpha subunit with a putative signal peptide of 24 amino acids and a mature protein of 96 amino acids. The cloned TSH beta cDNA consists of 580-bp nucleotides, including 67-bp of 5’UTR, 402-bp of the open reading frame, and 97-bp of 3’UTR followed by a 14 bp poly A trait. It encodes a putative precursor protein of 133 amino acids with signal peptide of 19 amino acids and a mature protein of 114 amino acids. The cloned FSH

並列關鍵字

GnRH ； gene expression and regulation ； subunit ； subunit ； diversity ； evolution ； lutropin ； TRH ； thyroid hormone ； phylogenetic tree ； thyrotropin ； follitropin ； pituitary glycoprotein hormone ； sinensis ； Chinese soft-shell turtle ； Pleodiscus

參考文獻

Aiyar, A., 2000. The use of CLUSTAL W and CLUSTAL X for multiple sequence alignment. Methods Mol. Biol. 132, 221-241.

Aizawa,Y., Ishii,S., 2003. Cloning of the cDNAs encoding the beta subunit precursor molecules of pituitary glycoprotein hormones in the Reeves's turtle (Geoclemys reevesii) and Japanese grass lizard (Takydromus tachydromoides). Gen. Comp. Endocrinol. 132, 465-473.

Ando, H. and Ishii, S., 1994. Molecular cloning of complementary deoxyribonucleic acids for the pituitary glycoprotein hormone alpha-subunit and luteinizing hormone beta-subunit precursor molecules of Japanese quail (Coturnix coturnix japonica). Gen. Comp. Endocrinol. 93 (3), 357-368.

d'Angelo-Bernard, G., Moumni, M., Jutisz, M., Counis, R., 1990. Cloning and sequence analysis of the cDNA for the precursor of the

國際替代計量

中華鱉 (Pelodiscus sinensis)腦垂體糖蛋白激素 cDNA序列mRNA生成調控之分析

全文下載

主題瀏覽