酸鹼濃度及化學表面修飾對於壓阻式微懸臂梁生物感測器之影響

由於醫療觀念逐漸被重視，所有相關的疾病檢測以及健康檢測也因應蓬勃發展，現今生物感測器大多仍使用化學或是螢光標記方式，而本實驗選擇以力學為基礎的微懸臂梁生物感測器做為量測機制，希望可以研發更具靈敏度的感測器。本實驗使用單根壓阻式微懸臂梁，希望可直接觀察微懸臂梁訊號並加以分析。微懸臂梁發展上常使用雙根微懸臂梁並配合惠司通電橋擷取訊號，雖然惠司通電橋在物理意義上能補償流體以及溫度對懸臂梁所造成的雜訊，但本研究發現由於雙根微懸臂梁表面的材料並不相同，容易造成化學訊號反應差異，故本實驗選擇單根壓阻式微懸臂梁做為研究機制，在適當的控溫下可將溫度限制變化在0.5oC之內。在進行化學表面修飾前後，探討不同pH值對於壓阻式微懸臂梁之影響。結果顯示，當pH值在2,3,11,12皆會對於微懸臂梁訊號產生反應，而pH值在4-10之間並不會使微懸臂梁訊號變化，當pH值較小時，微懸臂梁的訊號上升；反之，當pH值較大時，微懸臂梁的訊號下降。上述結果，有助於在檢測生物訊號時對於單根壓阻式微懸臂梁於酸鹼值影響上的掌握。本實驗已成功了解到微懸臂梁在酸鹼值下之反應範圍，以及當連結物鍵結鍵結在微懸臂梁後對於酸鹼值靈敏度降低，有了這些了解未來可以進行蛋白質檢測實驗，利用生物分子間的專一性鍵結，有效的測試及分析出生物分子對於訊號的影響，經過足夠量的分析統計過後，在遠程目標上，吾人希望可以結合無線傳輸的方式與本實驗的生物感測器做一完美性的結合。

關鍵字

壓阻式微懸臂梁；酸鹼；化學表面修飾

並列摘要

With the advancement of technology, relevant disease detection and health examination are growing with prosperity. However, most of the biological sensors are still using chemical or fluorescent markers as detectors, which are having lower sensitivities. This work utilized single-beam micro-cantilever biosensor for measuring biological signal, and for targeting the development of a more sensitive detecting method. In most of the studies double-beam micro-cantilever biosensors are constructed as two resistances of the Wheatstone bridge and one is taken as reference to compensate for background noises resulting from fluid field and temperature change. But this work discovered that the difference of surface materials of the two beams may result in ignoring effects of chemical reactions to biological signals. In the present study we select single free-beam devices as measuring mechanism to maintain the temperature change in 5oC. Utilizing immune analysis, the beam surface was treated by gold-sulfur bonding to explore the effect of surface modification (HS-(CH2)7-COOH) on micro cantilever beams. The results show that pH does make effect on the cantilevers at the values of 2, 3, 11, 12, while pH does not make effect on cantilevers at pH values between 4 to 10. The signals increase at smaller pH values and decrease at larger pH values. For results mentioned above, the unwanted signal arisen from the single-beam piezoresistive cantilever response to varying pH value can be eliminated in the detection of biological for future reference. We have successfully discovered the response to varying pH value effect on the single-beam micro cantilever and measured the decline of output signals after chemical adsorption.

並列關鍵字

piezoresistive microcantilever ； pH ； chemical surface modification

參考文獻

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酸鹼濃度及化學表面修飾對於壓阻式微懸臂梁生物感測器之影響

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