- 學位論文
高氮無鎳不銹鋼之機械性質與生物相容性研究
Study on Mechanical Properties and Biocompatibility of High Nitrogen Nickle-free stainless steel
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摘要
沃斯田鐵不銹鋼具有優良的性質，故常被作為骨科植入之用，但由於成分含鎳有毒性之慮，加上鎳價上漲使得煉鋼原料成本大漲，為了降低鎳的含量而可使用氮取代鎳元素。台大實驗室曾運用電漿熔煉探討研製高氮無鎳不銹鋼與製程參數之關係，利用錳來提高氮在沃斯田鐵裡的溶解度並求得最佳之合金成分及熔煉參數15Cr-22Mn-3Mo，因此本實驗以15Cr-22Mn-3Mo三種加錳無鎳的不同氮含量之高氮不銹鋼，並搭配市售316L不銹鋼作機械腐蝕性質上的比較加氮和錳的影響，然後也就針對細胞毒性反應、發炎反應和細胞黏附等實驗選擇Raw264.7巨噬細胞來進行評估高氮無鎳不銹鋼生物相容性。實驗結果證明材料硬度因含氮量的增加而提高，並且經由動態腐蝕電位測試儀配合模擬人體中的環境下使用細胞培養液，也可看出材料因氮含量提升而抗腐蝕能力提高，孔蝕電位也增加；材料與老鼠巨噬細胞Raw264.7培養模擬體外與細胞接觸的情形發現，細胞毒性測試顯示熔煉的三個合金其細胞存活率和商用316L相比並沒有顯著差異，發炎反應試驗材料不會引起巨噬細胞產生一氧化氮，但是熔煉的三個合金卻有引發ROS之慮；細胞黏附實驗方面，顯示合金粗糙表面有助於巨噬細胞黏附生長穩定。由實驗結果可看出藉由添加氮氣來強化材料其抗腐蝕性質的能力與錳和氮氣的綜合作用有關，耐腐蝕性質的提升有利於增加材料植入的生物相容性。
並列摘要
Due to the good properties of austenitic stainless steel, it is used for implanted orthopedic materials. But as result of the nickel'toxic potential and the nickel price rise causes the cost of steel-making rise sharply, we can replace the nickel content for nitrogen, and the manganese may enhance the solubility of nitrogen content in austenitic steel. Our laboratory once used the plasma melting to develop the relations between high nitrogen nickel-free stainless steel and melting parameter, and obtain the best alloying constituent and smelting parameter 15Cr-22Mn-3Mo. Therefore this research will aim at the different nitrogen contents of self-made ingot to discuss the machanical corrosion properties, and study on the alloy' biocompatibility with mouse macrophage RAW264.7. This experiment melt the 15Cr-22Mn-3Mo in ratio by plasma melting, , and take three ingot to proceed the dynamic electric potential polarization curve test, the cytotoxicity test, the cell inflammation test, as well as the cell adhesion ability to judge the material'availability degree. Experimental results support increase of hardness because of the increase of nitrogen content, and using the cell nutrient fluid by way of the dynamic corrosion potential test, also show that corrosion resistance ability enhance and pitting corrosion resistance do also. However, simulating the situation of material contacting the mouse macrophage raw264.7 demonstrate the three ingots survival percentages and commercial 316L compare do not make the big difference, and material do not cause the macrophage to produce the nitrogen monoxide, but have the potential to induce macrophage producing ROS. In cell adhesion test, demonstrate that the alloy's rough surface is helpful in the macrophage adhesion and be stable to growth. In brief, alloying Mn may degrade the resistance of the HNSSs to localized corrosion. The improvement of corrosion resistance caused by the addition of nitrogen could be attributed to the synergistic effects of nitrogen and manganese in the HNSSs.
參考文獻
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