以聚電解質多層膜修飾聚碸對血液相容性的影響

由於聚電解質多層膜具有易於製備，並且可適用於多種幾何形狀表面等優點，因此將此方法應用於生醫材料改質方面，提升原有的生物或血液相容性，具有相當潛力。血漿蛋白質在多層膜上吸附會受到多層膜表面性質影響，而纖維蛋白原是其中媒介血小板貼附最重要的角色。在聚電解質多層膜表面，預先吸附的蛋白質會影響血小板貼附與活化。除此之外，血小板的活化亦受到多層膜表面性質影響，多層膜表面的粗糙度、親水性及化學性質都可能直接或間接影響血小板的行為。由於聚碸已經是普遍使用的生醫材料，具有穩定的性質。本實驗選擇以聚碸為基材，經磺酸化後以聚電解質Poly(allylamine hydrochloride) (PAH)及Poly(acrylic acid) (PAA)，利用layer-by-layer方法修飾磺酸化聚碸。在使用pH 2.0與pH 6.5的聚電解質溶液，在不同層數下探討血小板的貼附與型態。使用pH 2.0聚電解質溶液製備的多層膜表面，多層膜層數增加並不影響表面纖維蛋白原的吸附量，然而在使用pH 6.5的聚電解質溶液製備的多層膜表面，隨著多層膜層數增加，纖維蛋白原的吸附量也提升。至於血小板貼附與型態的部分，在兩種酸鹼值的聚電解質溶液製備出的多層膜表面，隨著層數遞增，血小板的活化指數均隨之增加。整體而言，在以pH6.5的電解質溶液製備的多層膜表面，纖維蛋白質吸附量與血小板貼附量均高於以pH2.0電解質溶液製備的多層膜表面。不同pH值與不同層數的多層膜，對血小板貼附與活化程度的影響與其表面性質均有所關聯。

關鍵字

聚碸聚電解質多層膜；血液相容性；纖維蛋白原；血小板

並列摘要

Surface modification of biomaterials by polyelectrolyte layer-by-layer (LBL) deposition is a newly emerging method to modulate biocompatibility for biomedical devices. LBL deposition can be applied to biomedical devices with complicated geometries, which make it an attracting method. In this study we applied this method to improve blood compatibility of polysulfone (PSF). PSF, after sulfonation, was coated with multilayer of poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA) which was assembled at either pH 2.0 or pH 6.5 by LBL deposition to desired number of layers. The modified surfaces were characterized by atomic force microscope, contact angle measurement, XPS and FTIR. We found that platelet adhesion was low on the LBL surfaces which were prepared at pH 2.0, while platelet adhesion was increased with increasing number of layers when the LBL surfaces were prepared at pH 6.5. Platelet adhesion was correlated to the adsorption of fibrinogen, the most critical plasma protein mediating platelet adhesion. Furthermore, platelets activation was increased with the layer numbers. In conclusion, platelet adhesion and activation is modulated by the pH of deposition solution and the number of layers of polyelectrolyte multilayer.

並列關鍵字

Polysulfone ； polyelectrolyte multilayer ； blood compatibility ； fibrinogen ； platelet

參考文獻

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以聚電解質多層膜修飾聚碸對血液相容性的影響

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