菇蕈多醣體對腸道黏膜免疫系統之影響

The Effect of Mushroom Polysaccharides on Intestinal Mucosal Immune System

何淑螢(Shu-Ying Ho)

指導教授 ： 陳秀男

國立臺灣大學/生命科學院/漁業科學研究所/碩士(2013年)

https://doi.org/10.6342/NTU.2013.11187

摘要

菇蕈多醣體在動物體中具有免疫調節及輔助免疫反應之功能，並且促進多種免疫球蛋白表現；免疫球蛋白A（IgA）在腸道黏液中大量表現，由腸上細胞表現poly-Ig receptor參與的轉胞吞作用分泌至腸道中，針對病原體執行中和作用。本實驗欲探討多醣體對小鼠腸道黏膜免疫系統免疫球蛋白表現之影響，首先建立刺激小鼠腸道黏膜免疫系統之模式，再進一步探討以灌食方式給予小鼠多醣體，是否增加腸道免疫球蛋白A、免疫球蛋白G（IgG）及免疫球蛋白M（IgM）的表現量，以及對小腸組織poly-Ig receptor基因表現量之影響。結果顯示小腸清洗液中IgA以及IgG顯著提升，血清中IgG以及IgM表現也有提升的趨勢，發現以灌食途徑給予小鼠多醣體，首先活化小鼠腸道黏膜免疫系統，進而促進小鼠身體免疫反應。而協助腸道中免疫球蛋白分泌的poly-Ig receptor基因表現量也有提升的情形。

腸道黏膜免疫系統維持腸道環境的動態平衡，而經由本實驗發現，多醣體能增加腸道以及血清中免疫球蛋白表現，並且提升參與腸道中免疫球蛋白分泌的poly-Ig receptor基因表現量，推測多醣體增強腸道黏膜免疫系統，減低腸道受到外來病原感染的機會，以確保腸道正常地運作進行消化及營養吸收。

關鍵字

腸道黏膜免疫系統 ； 菇蕈多醣體 ； 免疫球蛋白A ； 免疫球蛋白G ； 免疫球蛋白M

並列摘要

Mushroom polysaccharides are distinguished as important immunostimulant in animal body. Administrate glucan to animal inducing different isotype immunoglobulin secretion. Immunoglobulin A is the major antibody in the intestinal mucus, and transcytosis of IgA across epithelia is mediated by the poly-Ig receptor. Neutralization is important protection mechanism against antigen by IgA in gut. In the present study simulate acting intestinal mucosal immune system. Further to study the effects of mushroom polysaccharide on immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM) concentration in serum and small intestine washing fluid (SIWF) and intestine tissue poly-immunoglobulin receptor (poly-Ig receptor) mRNA expression.
Feeding mice polysaccharide IgA and IgG in SIWF are increase significantly, and IgG and IgM in serum are also increase significantly. The results suggest that the effect of polysaccharides was induced at intestinal mucosa firstly, and induced body circulation immune response further. Poly-Ig receptor mRNA expression increase significantly, too.
Our study highlights the efficacious effect of mushroom polysaccharides increasing immunoglobulin concentration in intestinal tract and serum immunoglobulin concentration, and increase poly-Ig receptor mRNA expression in intestine tissue. Mushroom polysaccharide may stimulate intestinal mucosal immune system to protect the intestinal tract from being damaged by the bacterial over-population.

並列關鍵字

intestinal mucosal immune system ； mushroom polysaccharides ； immunoglobulin A ； immunoglobulin G ； immunoglobulin M

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