As the data rates increase into the multi-gigabit range, the effect of slot lines discontinuities on printed circuit board becomes non-negligible. In order to investigate the signal integrity issues due to the slot lines, this paper discusses and analysis the effect of slot lines discontinuities. Then list four kind of compensation of slot, and by the multi-slots circuit investigates signal integrity and crosstalk due to multi-slots. Some circuit boards have been fabricated and the time-domain reflection ( TDR ) instrument is employed to make qualitative and quantitatively comparison between measurement and analysis. These signal integrity issues can be better handled from the results of the above measurement.